PXD020481-1

PXD020481 is an original dataset announced via ProteomeXchange.

Title	Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks
Description	Delineating the extracellular interaction network of the cell surface proteotype (the surfaceome) is fundamental to understanding cellular signaling function in health and disease. Here, we developed LUX-MS, an optoproteomic technology that enables the spatiotemporal and proteome-wide study of surfaceome signaling architectures at nanoscale level without the need for genetic manipulation. A tunable, light-triggered singlet oxygen generator (SOG) based mechanism mediates in-situ proximity-tagging for subsequent mass spectrometry-based identification of acute protein interactions in their native cellular context. We applied LUX-MS to the characterization of surfaceome signaling structures engaged by antibodies, small molecule drugs, biologics and intact bacteriophages across organisms and in complex environments. Cell-type resolved dissection of intercellular communication using LUX-MS thereby revealed the molecular architecture of functional immunological signaling synapses in unprecedented detail. Altogether, LUX-MS enables facebooking of the social protein networks within surfaceome signaling architectures and provides an unprecedented molecular framework for the rational design of biomedical intervention strategies.
HostingRepository	PRIDE
AnnounceDate	2021-06-14
AnnouncementXML	Submission_2021-06-14_15:22:33.309.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maik Müller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Listeria monocytogenes serotype 4b; NCBI TaxID: 2065118; scientific name: Listeria phage A500; NCBI TaxID: 40522; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dihydroxylated residue; isotope labeled residue; deaminated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive HF; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-21 17:52:58	ID requested
⏵ 1	2021-06-14 15:22:33	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS

Bernd Wollscheid
contact affiliation	Institute of Translational Medicine, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland
contact email	wbernd@ethz.ch
lab head
Maik Müller
contact affiliation	ETH Zurich
contact email	maimuell@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD020481

PRIDE project URI

• PRIDE
  1. PXD020481
     1. Label: PRIDE project
     2. Name: Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks