PXD020469-1

PXD020469 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of the developing heart identifies PERM1 - an outer mitochondrial membrane protein
Description	Heart development relies on PTMs that control cardiomyocyte proliferation, differentiation and cardiac morphogenesis. We generated a map of phosphorylation sites during the early stages of cardiac postnatal development in mice; we quantified over 10,000 phosphorylation sites and 5000 proteins that were assigned to different pathways. Analysis of mitochondrial proteins led to the identification of PGC-1- and ERR-induced regulator in muscle 1 (PERM1), which is specifically expressed in skeletal muscle and heart tissue and associates with the outer mitochondrial membrane. We demonstrate PERM1 is subject to rapid changes mediated by the UPS through phosphorylation of its PEST motif by casein kinase 2. Ablation of Perm1 in mice results in reduced protein expression of lipin-1 accompanied by accumulation of specific phospholipid species. Isolation of Perm1-deficient mitochondria revealed significant downregulation of mitochondrial transport proteins for amino acids and carnitines, including SLC25A12/13/29/34 and CPT2. Consistently, we observed altered levels of various lipid species, amino acids and acylcarnitines in Perm1-/- mitochondria. We conclude that the outer mitochondrial membrane protein PERM1 regulates homeostasis of lipid and amino acid metabolites in mitochondria.
HostingRepository	MassIVE
AnnounceDate	2021-05-05
AnnouncementXML	Submission_2021-05-05_05:52:46.606.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastian Kallabis
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-21 04:20:06	ID requested
⏵ 1	2021-05-05 05:52:47	announced

Aravamudhan S, T, ü, rk C, Bock T, Keufgens L, Nolte H, Lang F, Krishnan RK, K, ö, nig T, Hammerschmidt P, Schindler N, Brodesser S, Rozsivalova DH, Rugarli E, Trifunovic A, Br, ü, ning J, Langer T, Braun T, Kr, ü, ger M, Phosphoproteomics of the developing heart identifies PERM1 - An outer mitochondrial membrane protein. J Mol Cell Cardiol, 154():41-59(2021) [pubmed]

submitter keyword: Heart development, Phosphoproteomics, SILAC, Mitochondria, PERM1, Lipid Metabolism

Marcus Krueger
contact affiliation	CECAD Research Center, Institute for Genetics, University of Cologne, Joseph-Stelzmann-Str. 26, 50931 Cologne
contact email	marcus.krueger@uni-koeln.de
lab head
Sebastian Kallabis
contact affiliation	CECAD
contact email	skallab5@uni-koeln.de
dataset submitter

MassIVE dataset URI

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