PXD020441-2

PXD020441 is an original dataset announced via ProteomeXchange.

Title	Global Phosphoproteomics of DYRK1A Inhibition in Glioblastoma Cells
Description	Here we performed a detailed investigation to delineate the role of DYRK1A in glioblastoma, using both pharmacological and genetic tools combined with global phosphoproteomics. Our data show that DYRK1A inhibitors affect a much broader proportion of the phosphoproteome than DYRK1A knockdown. By overlaying these datasets we identify a pool of 61 putative novel DYRK1A targets, and validate CDC23 Ser588 as a bona-fide DYRK1A substrate. CDC23 is a ubiquitin ligase that degrades mitotic proteins, and DYRK1A inhibiton thereby leads to the accumulation of cyclin B and activation of CDK1.
HostingRepository	PRIDE
AnnounceDate	2021-04-22
AnnouncementXML	Submission_2021-04-21_23:22:48.262.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sean Humphrey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-20 01:04:21	ID requested
1	2021-04-21 22:08:52	announced
⏵ 2	2021-04-21 23:22:49	announced	2021-04-22: Updated project metadata.
3	2024-10-22 05:21:30	announced	2024-10-22: Updated project metadata.

10.1038/S41420-021-00456-6;

Recasens A, Humphrey SJ, Ellis M, Hoque M, Abbassi RH, Chen B, Longworth M, Needham EJ, James DE, Johns TG, Day BW, Kassiou M, Yang P, Munoz L, Global phosphoproteomics reveals DYRK1A regulates CDK1 activity in glioblastoma cells. Cell Death Discov, 7(1):81(2021) [pubmed]

submitter keyword: glioblastoma, proteomics, U251, DYRK1A, phosphoproteome, post-translational modification, mass spectrometry, signal transduction, phosphorylation,cancer, phosphoproteomics

Sean Humphrey
contact affiliation	Charles Perkins Centre, The University of Sydney
contact email	sean.humphrey@sydney.edu.au
lab head
Sean Humphrey
contact affiliation	Sydney University
contact email	sean.humphrey@sydney.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD020441

PRIDE project URI

• PRIDE
  1. PXD020441
     1. Label: PRIDE project
     2. Name: Global Phosphoproteomics of DYRK1A Inhibition in Glioblastoma Cells