⮝ Full datasets listing

PXD020434-1

PXD020434 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAllosteric priming of E. coli CheY by the flagellar motor protein FliM
DescriptionWe detail simulations and solution measurements to better understand the differences between the native and D13K-Y106W CheY crystal structures. We resolved the complex conformational landscapes by MD simulations with mutual information measures to determine the coupling between protein fragments. Protection experiments with XFMS (X-ray foot-printing with mass spectroscopy), a technique that probed sidechain solvent accessibility in contrast to deuterium exchange of backbone hydrogen atoms, supported the FliMN requirement for D13K-Y106W CheY activation reported by the crystal structures, and the MD allosteric network model. XFMS has a more straight-forward physical rationale than fluorescence quenching for reporting sidechain motions over time-resolved windows and is not limited by the size of the protein assembly. Further analysis of the MD trajectories resolved multiple CheY Y106 rotamer states. Inward orientation was temporally coupled to stabilization of both the CheY fold and the FliMN interface in the CheY·FliMN complex, but not in CheY alone. The coupling increased in D13K-Y106WCheY·FliMN. The formation of a distinct module that orchestrates CheY dynamicsto stabilize new surface topologies for possible second-stage binding to FliN was the signature of the fully activated D13K-Y106W CheY·FliMN state.
HostingRepositoryPRIDE
AnnounceDate2020-09-21
AnnouncementXMLSubmission_2020-09-20_22:45:27.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD020434
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristopher Petzold
SpeciesList scientific name: Escherichia coli BL21(DE3); NCBI TaxID: 469008;
ModificationListdihydroxylated residue; monohydroxylated residue
Instrument6550 iFunnel Q-TOF LC/MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-19 22:01:26ID requested
12020-09-20 22:45:28announced
22024-10-22 05:12:32announced2024-10-22: Updated project metadata.
Publication List
Wheatley P, Gupta S, Pandini A, Chen Y, Petzold CJ, Ralston CY, Blair DF, Khan S, coli CheY by the Flagellar Motor Protein FliM. Biophys J, 119(6):1108-1122(2020) [pubmed]
Keyword List
submitter keyword: CheY, FliM, XFMS, Atomistic simulation, Flagellar
Contact List
Christopher J. Petzold
contact affiliationStaff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact emailcjpetzold@lbl.gov
lab head
Christopher Petzold
contact affiliationLawrence Berkeley National Laboratory
contact emailcjpetzold@lbl.gov
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD020434
PRIDE project URI
Repository Record List
[ + ]