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PXD020426-2

PXD020426 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHeterotypic signaling between dermal fibroblasts and melanoma cells induces phenotypic plasticity and proteome rearrangement in malignant cells
DescriptionThe signaling events triggered by soluble mediators released from both transformed and stromal cells shape the phenotype of tumoral cells and have significant implications in cancer development and progression. In this study we performed an in vitro heterotypic signaling assay by evaluating the proteome diversity of human dermal fibroblasts after stimulation with the conditioned media obtained from malignant melanoma cells. In addition, we also evaluated the changes in the proteome of melanoma cells after stimulation with their own conditioned media as well as with the conditioned medium from melanoma-stimulated fibroblasts. Our results pointed out to a significant rearrangement in the proteome of stromal and malignant cells upon crosstalk of soluble mediators. The main proteome signature of stimulated cells was related to protein synthesis, which may indicate that this process might be an early response of stimulated stromal cells. In addition, the conditioned medium derived from ‘primed’ stromal cells (melanoma-stimulated fibroblasts) was more effective in altering the functional phenotype (cell migration) of malignant cells than the fibroblast conditioned medium alone. Collectively, self- and cross-stimulation may play a key role in shaping the tumor microenvironment and, more importantly, enable tumoral cells to succeed in the process of melanoma progression and metastasis. Although the proteome landscape of cells participating in such a heterotypic signaling represents a snapshot of a highly dynamic state, understanding the diversity of proteins and enriched biological pathways resulting from stimulated cell states may allow for targeting specific cell regulatory motifs involved in melanoma progression and metastasis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:11:33.319.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndre Zelanis
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-18 16:25:54ID requested
12020-08-30 22:36:21announced
22024-10-22 05:11:33announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: cancer-associated fibroblasts,heterotypic signaling, fibroblast, phenotypic plasticity., melanoma
Contact List
André Zelanis
contact affiliationFunctional Proteomics Laboratory Department of Science and Technology Federal University of São Paulo (ICT-UNIFESP) São José dos Campos, São Paulo, Brazil Rua Talim, 330, 12231-280; Tel +55 11 3385-4135 ext. 9727.
contact emailandre.zelanis@unifesp.br
lab head
Andre Zelanis
contact affiliationLaboratory of Functional Proteomics - Department of Science and Technology - Federal University of São Paulo (UNIFESP), Sao Jose dos Campos-SP, Brazil
contact emailzelanis@gmail.com
dataset submitter
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