⮝ Full datasets listing

PXD020403-1

PXD020403 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMitochondrial defects contribute to impaired translational in SMA motor neurons
DescriptionSpinal muscular atrophy (SMA) is a neuromuscular disease, characterized by loss of lower alpha motoneurons, which leads to proximal muscle weakness. SMA is caused by reduced levels of Survival of Motor Neuron protein due to biallelic deletions or mutations in the SMN1 gene. Dysfunctional mitochondria can harm cells by decreased ATP production, but also by increased oxidative stress due to elevated production of reactive oxygen species (ROS). In this study, whole proteome analysis was performed using the Taiwanese SMA mouse model on an FVB/N background to identify changes in the proteome related to oxidative stress. Therefore, primary WT and SMA motoneurons were treated with a known anti-oxidant N-Acetylcysteine, the ROS inducer menadione and the cell culture supplement sodium pyruvate. Furthermore, this study aims to investigate changes in mRNA translation initiation affected by oxidative stress in SMA.
HostingRepositoryPRIDE
AnnounceDate2020-12-23
AnnouncementXMLSubmission_2020-12-23_01:51:03.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaximilian Thelen
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-16 07:27:33ID requested
12020-12-23 01:51:04announced
22021-03-09 23:13:59announced2021-03-10: Updated publication reference for PubMed record(s): 33353564.
32024-10-22 05:17:03announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Spinal muscular atrophy, mitochondria, reactive oxygen species, initiation of mRNA translation, SMN
Contact List
Min Jeong Kye
contact affiliationInstitute of human genetics, University of cologne
contact emailmin.kye@uk-koeln.de
lab head
Maximilian Thelen
contact affiliationInstitute of human genetics, Cologne
contact emailmaximilian.thelen@uk-koeln.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD020403
PRIDE project URI
Repository Record List
[ + ]