PXD020383 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Lineage-Specific Proteomic Signatures in the Mycobacterium Tuberculosis Complex Reveal Differential Abundance of Proteins Involved in Virulence, DNA Repair, CRISPR-Cas, Bioenergetics and Lipid Metabolism |
Description | Despite the discovery of Mycobacterium tuberculosis (Mtb) more than 130 years ago, Mtb physiology and the mechanisms of virulence are still not fully understood. The objective of this study was to compare and characterize the differentially abundant protein profiles of modern, pre-modern and ancient Mtb lineages. Using a comprehensive analysis of the proteome of Mtb lineages 3, 4, 5 and 7, unique and shared proteomic signatures in these modern, pre-modern and ancient Mtb lineages were delineated. Main proteomic findings were verified by using immunoblotting. In addition, analysis of multiple genome alignment of all lineages was performed using Geneious Prime software. Label-free peptide quantification of whole cells from Mtb lineage 3, 4, 5 and 7 yielded 38,346 unique peptides derived from 3092 proteins, representing 77% coverage of the predicted Mtb proteome. Mtb lineage-specific differential abundances was observed for 539 proteins. Lineage 7 exhibited a markedly reduced abundance of proteins involved in DNA repair, type VII ESX-3 and ESX-1 secretion systems, lipid metabolism and inorganic phosphate uptake, and an increased abundance of proteins involved in alternative pathways of the TCA cycle and the CRISPR/Cas system as compared to the other lineages. Lineages 3 and 4 exhibited a higher abundance of proteins involved in virulence, DNA repair, drug resistance and other metabolic pathways. The high throughput analysis of Mtb proteome by super resolution massspectrometry provided an insight into the differential expression of proteins between Mtb lineages 3, 4, 5 and 7 that may explain the slow growth and reduced virulence of lineage 7, as well as metabolic flexibility and the ability to survive under adverse growth conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2020-09-01 |
AnnouncementXML | Submission_2020-09-01_04:44:15.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tahira Riaz |
SpeciesList | scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773; |
ModificationList | acetylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-15 07:45:31 | ID requested | |
⏵ 1 | 2020-09-01 04:44:15 | announced | |
2 | 2020-10-21 23:56:34 | announced | 2020-10-22: Updated publication reference for PubMed record(s): 33072011. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Mycobacterium tuberculosis |
lineage 7, proteomics, DNA repair, ESX-3 secretion system, virulence, Ethiopia |
Contact List
Tone Tønjum |
contact affiliation | University of Oslo, Norway |
contact email | tone.tonjum@medisin.uio.no |
lab head | |
Tahira Riaz |
contact affiliation | University of Oslo |
contact email | tahira.riaz@medisin.uio.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020383
- Label: PRIDE project
- Name: Lineage-Specific Proteomic Signatures in the Mycobacterium Tuberculosis Complex Reveal Differential Abundance of Proteins Involved in Virulence, DNA Repair, CRISPR-Cas, Bioenergetics and Lipid Metabolism