PXD020359-1

PXD020359 is an original dataset announced via ProteomeXchange.

Title	Isolation and mass spectrometry analysis of Atg9 vesicles from Saccharomyces cerevisiae
Description	Autophagy is a eukaryotic bulk degradation pathway that allows cells to degrade potentially harmful cytosolic components or to provide necessary nutrients during starvation. This cargo degradation is achieved by its sequestration within double membrane vesicles termed autophagosomes, which fuse with the vacuole where it is degraded. Atg (autophagy-related) proteins are the main group of proteins involved in this process and, interestingly, Atg9 is the only integral membrane yeast Atg protein absolutely required for autophagy progression. In the cell it resides in Golgi-derived vesicles, which are indispensable for the nucleation of the autophagosome. There is not much known about biochemical properties of these vesicles in particular their protein content apart from Atg9. To address this question and to identify putative interaction partners of Atg9, the Atg9-vesicles were isolated and submitted to mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2022-03-03
AnnouncementXML	Submission_2022-03-03_00:45:51.122.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Hartl
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-14 05:02:08	ID requested
⏵ 1	2022-03-03 00:45:52	announced

Sawa-Makarska J, Baumann V, Coudevylle N, von B, ü, low S, Nogellova V, Abert C, Schuschnig M, Graef M, Hummer G, Martens S, Reconstitution of autophagosome nucleation defines Atg9 vesicles as seeds for membrane formation. Science, 369(6508):(2020) [pubmed]

submitter keyword: yeast, autophagy, Atg9, vesicles

Sascha Martens
contact affiliation	Max Perutz Labs Vienna, Department of Biochemistry and Cell Biology, University of Vienna, Vienna BioCenter, Austria
contact email	sascha.martens@univie.ac.at
lab head
Markus Hartl
contact affiliation	Mass Spectrometry Facility, Max Perutz Labs Vienna, University of Vienna, Vienna Biocenter, Dr.-Bohr-Gasse 3, 1030 Vienna
contact email	markus.hartl@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD020359

PRIDE project URI

• PRIDE
  1. PXD020359
     1. Label: PRIDE project
     2. Name: Isolation and mass spectrometry analysis of Atg9 vesicles from Saccharomyces cerevisiae