PXD020335-1

PXD020335 is an original dataset announced via ProteomeXchange.

Title	Inhibition of human glioblastoma invasion and growth upon co-administration of a synergic miRNA pool actuated in vivo by nanocarriers
Description	Although small interfering RNAs are being routinely used in the clinic, the therapeutic potential of oncogene co-regulation via multiple microRNAs (miRNAs) is still vastly underexplored. Here, the functional synergism of a pool of 11 miRNAs, which are involved in neural stem cell differentiation, was investigated in 3 different patient-derived GBM clones as well as in a conventional orthotopic mouse model of human GBM. First, we confirmed that the 11 miRNAs are mostly downregulated in all tested human GBM cells. Then, we demonstrated that the modulation of the 11-miRNA pool affected the expression of proteins involved in tumor cell adhesion and glioma stemness. These findings positively correlate with a significant reduction in cancer cell invasiveness, which was documented in both cell monolayer and tumor spheroid assays. Notably, a single miRNA of the 11 (i.e., miR-124) cannot recapitulate the functional synergism documented with the pool. Moreover, the 11 miRNAs downregulated an identical subset of proteins in different patient-derived GBM cells, several of them involved in the Collagen pathway, that correlate with the malignancy grade and subtype, further supporting the ability of the selected pool to modulate tumor aggressiveness. Finally, the 11-miRNA pool was efficiently encapsulated into Apolipoprotein E-coated lipid nanoparticles and deposited in orthotopic murine models of human GBM. A single nanoparticle administration significantly impaired tumor growth and extended survival by >50% compared to control experiments, without inducing any visible adverse effect. This study highlights the role of miRNAs’ functional synergism in treating malignancies with dismal prognosis and proves that nanomedicines can be used to effectively deliver multiple, different miRNAs in vivo, possibly in combination with chemotherapeutic drugs.
HostingRepository	PRIDE
AnnounceDate	2026-01-05
AnnouncementXML	Submission_2026-01-04_17:26:25.923.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Armirotti
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-13 04:29:40	ID requested
⏵ 1	2026-01-04 17:26:27	announced

Rancati S, Pereira RC, Schlich M, Sgroi S, Beatini S, La Rosa L, Giantomasi L, Pelizzoli R, Braccia C, Di Fonzo A, Spattini C, Tuntevski K, Lo Van A, Pons-Espinal M, Palange A, Bajetto A, Daga A, Armirotti A, Florio T, Decuzzi P, De Pietri Tonelli D, Synergic microRNAs suppress human glioblastoma progression by modulating clinically relevant targets. Mol Ther Nucleic Acids, 36(4):102763(2025) [pubmed]

10.1016/j.omtn.2025.102763;

submitter keyword: nanoformulation, miRNA,GLioblastoma

Andrea Armirotti
contact affiliation	Analytical Chemistry Lab Fondazione Istituto Italiano di Tecnologia Via Morego 30, 16163 Genova Italy
contact email	andrea.armirotti@iit.it
lab head
Andrea Armirotti
contact affiliation	Analytical Chemistry Lab, Fondazione Istituto Italiano di Tecnologia
contact email	andrea.armirotti@iit.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020335
     1. Label: PRIDE project
     2. Name: Inhibition of human glioblastoma invasion and growth upon co-administration of a synergic miRNA pool actuated in vivo by nanocarriers