PXD020326-2

PXD020326 is an original dataset announced via ProteomeXchange.

Title	Quantification of histone H3 and H4 modifications by mass spectrometry in wildtype and MCM2-2A mutants of mice ESCs
Description	During DNA replication modified parental histones H3-H4 are segregated almost symmetrically to the two daughter DNA strands enabling mitotic inheritance of histone PTMs. Whether heritable histone modifications confer epigenetic regulation by maintaining chromatin states and gene expression is unclear. Using MCM2 histone-binding mutant embryonic stem cells (ESCs) and mass spectrometry, we show that asymmetric segregation of parental histones to the leading strand causes imbalanced inheritance of histone H3K27 methylations to daughter cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:02:14.801.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Victor Solis
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylated lysine; N6-acetyl-L-lysine; N6,N6-dimethyl-L-lysine; N6,N6,N6-trimethyl-L-lysine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-13 00:51:36	ID requested
1	2024-10-07 13:34:29	announced
⏵ 2	2024-10-22 06:02:15	announced	2024-10-22: Updated project metadata.

10.1038/S41588-023-01476-X;

submitter keyword: H3 acetylation, H4 methylation, histones, H3 methylation, ESCs,MCM2, MCM2-2A

Anja Groh
contact affiliation	Novo Nordisk Foundation Center for Protein Research (CPR), University of Copenhagen, Copenhagen, Denmark.
contact email	anja.groth@cpr.ku.dk
lab head
Victor Solis
contact affiliation	EpiQMAx GmbH
contact email	solis@genzentrum.lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD020326

PRIDE project URI

• PRIDE
  1. PXD020326
     1. Label: PRIDE project
     2. Name: Quantification of histone H3 and H4 modifications by mass spectrometry in wildtype and MCM2-2A mutants of mice ESCs