PXD020268-1

PXD020268 is an original dataset announced via ProteomeXchange.

Title	DNAJC9, a dual histone and heat shock co-chaperone for histone transactions
Description	Nucleosomes are assembled and disassembled in a highly regulated manner by histone chaperones, which shield histones from non-specific interactions (Hammond, Strømme et al. 2017). From biosynthesis to deposition histones are handed through a cascade of histone chaperones, yet it remains unclear how cells control histone transfer reactions and cleanup spurious interactions. We identified DNAJC9 as a novel histone chaperone that directs protein folding machinery to histone substrates, promoting their entry/re-entry into the supply chain and release from trapped intermediates. DNAJC9 recruits HSP70 catalysis to its histone H3–H4 cargo via its conserved J domain. We elucidate the structure of DNAJC9 in a histone H3–H4 co-chaperone complex with MCM2, revealing how the dual histone and heat shock co-chaperone binds histone substrates. Failure to engage DNAJC9 causes histones to accumulate upstream in the histone supply chain prior to H3–H4 heterodimer formation. In addition, DNAJC9 mutations that abolish HSP70 mediated catalysis traps histones with DNAJC9 shortly after histone biosynthesis and during replication- and transcription-coupled nucleosome assembly. Together, DNAJC9 combines both histone and heat shock co-chaperone abilities to direct HSP70 catalysis towards histone substrates facilitating their supply to and transactions within chromatin.
HostingRepository	PRIDE
AnnounceDate	2021-04-14
AnnouncementXML	Submission_2021-04-14_05:28:29.801.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivo Hendriks
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-09 02:02:34	ID requested
⏵ 1	2021-04-14 05:28:30	announced
2	2021-04-18 23:15:43	announced	2021-04-19: Updated project metadata.
3	2022-02-14 02:19:44	announced	2022-02-14: Updated project metadata.
4	2024-10-22 05:21:09	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: DNAJC9, HSP70, HSP40, histone chaperone, chaperone, heat shock

Michael Lund Nielsen
contact affiliation	Proteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark
contact email	michael.lund.nielsen@cpr.ku.dk
lab head
Ivo Hendriks
contact affiliation	Novo Nordisk Foundation Center for Protein Research, University of Copenhagen
contact email	ivo.a.hendriks@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD020268

PRIDE project URI

• PRIDE
  1. PXD020268
     1. Label: PRIDE project
     2. Name: DNAJC9, a dual histone and heat shock co-chaperone for histone transactions