PXD020260-2

PXD020260 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics Identifies Sytenin-1 as a Universal Biomarker of Exosomes
Description	Exosomes are extracellular vesicles derived from the endosomal compartment. They contain a multitude of bioactive constituents and mediate intercellular communication in health and disease. The frequently used biomarkers of exosomes are heterogeneous and do not exhibit universal utility across different cell types. To uncover ubiquitous and abundant proteins that can serve as universal biomarkers of exosomes, we employed an unbiased and quantitative proteomic approach based on Super SILAC coupled to high-resolution mass spectrometry (MS). In total 1,243 proteins were consistently quantified in the proteome of exosomes, irrespective of the cellular source or isolation method. Among them, a cohort of 22 proteins were universally enriched, and 15 proteins consistently depleted in the proteome of exosomes when compared to cells. Among the enriched proteins, we found several membrane proteins and GTPases, whereas the cohort of depleted proteins was predominantly composed by nuclear proteins. Our study revealed that Syntenin-1 is the most abundant ubiquitous protein in exosomes from distinct cell sources, thus representing an ideal candidate for universal biomarker of exosomes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:22:51.406.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kelly Hodge
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-08 06:36:12	ID requested
1	2021-06-10 02:49:51	announced
⏵ 2	2024-10-22 05:22:51	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: LC-MS,exosomes, Super SILAC, Biomarker, Quantitative Proteomics

Sara Zanivan
contact affiliation	Head of Proteomics, CRUK Beatson Institute, UK
contact email	s.zanivan@beatson.gla.ac.uk
lab head
Kelly Hodge
contact affiliation	CRUK Beatson Institute
contact email	k.hodge@beatson.gla.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020260
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics Identifies Sytenin-1 as a Universal Biomarker of Exosomes