PXD020232-3

PXD020232 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Phosphoproteome of five-minute KCl-depolarised mouse cultured hippocampal neurons
Description	Cultured hippocampal neurons from mice were treated with high concentration KCl for five minutes to depolarise the plasma membrane and model sustained brain activity. A high KCl condition was compared to low KCl using phosphoproteomics. Phosphopeptides were enriched from trypsin digested bio-replicates of the high and low KCl conditions and analysed by mass spectrometry. Approximately 20,000 phosphopeptides were identified enabling a comparison of the relative level of phosphorylation in response to depolarisation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:05:59.620.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020232
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mark Graham
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-07 04:40:06	ID requested
1	2023-10-15 16:07:32	announced
2	2024-03-05 03:56:11	announced	2024-03-05: Updated project metadata.
⏵ 3	2024-10-22 06:06:00	announced	2024-10-22: Updated project metadata.

Publication List

10.1038/s41467-023-43130-4;
10.6019/PXD020232;
Longfield SF, Mollazade M, Wallis TP, Gormal RS, Joensuu M, Wark JR, van Waardenberg AJ, Small C, Graham ME, Meunier FA, Mart, í, nez-M, á, rmol R, Tau forms synaptic nano-biomolecular condensates controlling the dynamic clustering of recycling synaptic vesicles. Nat Commun, 14(1):7277(2023) [pubmed]

10.1038/s41467-023-43130-4;

10.6019/PXD020232;

Longfield SF, Mollazade M, Wallis TP, Gormal RS, Joensuu M, Wark JR, van Waardenberg AJ, Small C, Graham ME, Meunier FA, Mart, í, nez-M, á, rmol R, Tau forms synaptic nano-biomolecular condensates controlling the dynamic clustering of recycling synaptic vesicles. Nat Commun, 14(1):7277(2023) [pubmed]

Keyword List

submitter keyword: neurons, depolarisation, phosphoproteome,Mouse, hippocampal, tau, KCl, LC-MS/MS

submitter keyword: neurons, depolarisation, phosphoproteome,Mouse, hippocampal, tau, KCl, LC-MS/MS

Contact List

Mark Evan Graham
contact affiliation	Synapse Proteomics, Children’s Medical Research Institute, The University of Sydney, 214 Hawkesbury Road Westmead NSW 2145 Australia.
contact email	mgraham@cmri.org.au
lab head
Mark Graham
contact affiliation	Children's Medical Research Institute
contact email	mgraham@cmri.org.au
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD020232
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD020232

PRIDE project URI

Repository Record List

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