PXD020221-2

PXD020221 is an original dataset announced via ProteomeXchange.

Title	Quantitative mapping of proteome dynamics during polarization of human iPSC-derived neurons
Description	Early neuronal development is a well-coordinated process in which neuronal stem cells differentiate into polarized neurons. This process has been well studied in classical non-human model systems, but to what extent this is recapitulated in human neurons remains unclear. To study neuronal polarization in human neurons, we cultured human iPSC-derived neurons, characterized early developmental stages, measured electrophysiological responses, and systematically profiled transcriptomic and proteomic dynamics during these steps. We found extensive remodeling of the neuron transcriptome and proteome, with altered mRNA expression of ~1,100 genes and different expression profiles of ~1,500 proteins during neuronal differentiation and polarization. We also identified a distinct stage in axon development marked by an increase in microtubule remodeling and apparent relocation of the axon initial segment from the distal to proximal axon. Our comprehensive characterization and quantitative map of transcriptome and proteome dynamics provides a solid framework for studying polarization in human neurons.
HostingRepository	PRIDE
AnnounceDate	2020-09-18
AnnouncementXML	Submission_2020-10-04_23:43:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robbelien Kooistra
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-06 06:03:41	ID requested
1	2020-09-18 03:37:17	announced
⏵ 2	2020-10-04 23:43:49	announced	2020-10-05: Updated publication reference for PubMed record(s): 32940601.

Lindhout FW, Kooistra R, Portegies S, Herstel LJ, Stucchi R, Snoek BL, Altelaar AM, MacGillavry HD, Wierenga CJ, Hoogenraad CC, Quantitative mapping of transcriptome and proteome dynamics during polarization of human iPSC-derived neurons. Elife, 9():(2020) [pubmed]

submitter keyword: human neurons, hiPSC, NPC, differentiation

Casper Hoogenraad
contact affiliation	Cell biology, neurobiology and neurophysics, Utrecht University, Utrecht, Netherlands
contact email	c.hoogenraad@uu.nl
lab head
Robbelien Kooistra
contact affiliation	Utrecht University
contact email	r.kooistra@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD020221

PRIDE project URI

• PRIDE
  1. PXD020221
     1. Label: PRIDE project
     2. Name: Quantitative mapping of proteome dynamics during polarization of human iPSC-derived neurons