PXD020210-2

PXD020210 is an original dataset announced via ProteomeXchange.

Title	A Protein Kinase G -interacting protein regulates critical calcium signals in malaria parasites (Co-IP)
Description	In malaria parasites, cGMP signalling is mediated by a single cGMP-dependent protein kinase (PKG) . One of the major functions of PKG is to control calcium signals essential for the parasite to exit red blood cells or for the transmission of the gametocyte stages to the mosquito . However, how PKG controls these signals in the absence of known second messenger-dependent calcium channels or scaffolding proteins remains a mystery. Here we use pull-down approaches to identify a PKG partner protein in both Plasmodium falciparum schizonts and P. berghei gametocytes. This partner, named ICM1, is a polytopic membrane protein with homologies to transporters and calcium channels, raising the possibility of a direct functional link between PKG and calcium homeostasis. Phosphoproteomic analyses in both Plasmodium species highlight a densely phosphorylated region of ICM1 with multiple phosphorylation events dependent on PKG activity. Conditional disruption of the P. falciparum ICM1 gene results in reduced cGMP-dependent calcium mobilisation associated with defective egress and invasion. Stage-specific depletion of ICM1 in P. berghei gametocytes blocks gametogenesis due to the inability of mutant parasites to mobilise intracellular calcium upon PKG activation. These results provide us with new insights into the atypical calcium homeostasis in malaria parasites.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:18:03.836.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020210
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	alexandre Hainard
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-06 00:57:00	ID requested
1	2021-01-24 22:31:20	announced
⏵ 2	2024-10-22 05:18:04	announced	2024-10-22: Updated project metadata.

10.6019/PXD020210;

submitter keyword: gametocytes, LC-MSMS,plasmodium, co-IP

Mathieu Brochet
contact affiliation	Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, CH-1211 Switzerland
contact email	mathieu.brochet@unige.ch
lab head
alexandre Hainard
contact affiliation	University of Geneva
contact email	alexandre.hainard@unige.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020210
     1. Label: PRIDE project
     2. Name: A Protein Kinase G -interacting protein regulates critical calcium signals in malaria parasites (Co-IP)