PXD020197-1

PXD020197 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of brain mitochondrial-derived vesicles
Description	The generation of mitochondrial-derived vesicles (MDVs) is implicated in a plethora of vital cell functions, from mitochondrial quality control to peroxisomal biogenesis. The discovery of distinct subtypes of MDVs has revealed the selective inclusion of mitochondrial cargo in response to varying stimuli. However, the true scope and variety of MDVs is currently unclear, and unbiased approaches have yet to be used to understand their biology. Furthermore, as mitochondrial dysfunction has been implicated in many neurodegenerative diseases, it is essential to understand MDV pathways in the nervous system. To address this, we sought to identify the cargo in brain MDVs. We used an in vitro budding assay and proteomic approach to identify proteins selectively enriched in MDVs. 72 proteins were identified as MDV enriched, of which 31% were OXPHOS proteins. Interestingly, the OXPHOS proteins localized to specific modules of the respiratory complexes, hinting at the inclusion of sub-assemblies in MDVs. Small TIM chaperones were also highly enriched in MDVs, linking mitochondrial chaperone-mediated protein transport to MDV formation. As the two Parkinson’s disease genes PINK1 and Parkin have been previously implicated in MDV biogenesis in response to oxidative stress, we compared the MDV proteomes from the brains of wild-type mice with those of PINK1-/- and Parkin-/- mice. No significant difference was found, suggesting that PINK1- and Parkin-dependent MDVs make up a small proportion of all MDVs in the brain. Our findings demonstrate a previously uncovered landscape of MDV complexity and provide a foundation from which to discover further novel MDV functions.
HostingRepository	PRIDE
AnnounceDate	2020-09-07
AnnouncementXML	Submission_2020-09-07_01:33:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rosalind Roberts
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	hydroxylated residue; acetylated residue; monohydroxylated residue; nitrated L-tyrosine; nitrosylation; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-05 23:17:40	ID requested
⏵ 1	2020-09-07 01:33:31	announced
2	2024-10-22 05:11:52	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mouse, brain, LC-MS/MS, TimsTOF

Edward A Fon
contact affiliation	Montreal Neurological Institute, McGill University, Canada
contact email	ted.fon@mcgill.ca
lab head
Rosalind Roberts
contact affiliation	McGill University
contact email	rosalindfroberts@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020197
     1. Label: PRIDE project
     2. Name: Proteomic profiling of brain mitochondrial-derived vesicles