PXD020195 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Creation of a Candida albicans spectral library by Data Dependent Acquisition (DDA) |
Description | The incidence of candidiasis, caused mainly by the opportunistic fungal Candida albicans, remains a major concern in public health due to the emerging antifungal resistance. C. albicans modulates their proteome under different conditions trying to cope with several stressors in host niches. In this study we developed a C. albicans spectral library by analysing a total of 52 samples corresponding to cells treated with 5 and 10 mM hydrogen peroxide, 40 and 60 mM acetic acid and in the interaction with human macrophages (THP-1) at 3, 6 and 9 h (4 biological replicates). This samples represented several morphologies of C. albicans (yeast and hypha) and different culture conditions (temperature, media…) that contributed to the incorporation of different proteins into the library. The analysis of these conditions by DDA mode allowed the construction of a C. albicans spectral library comprising information of 2879 proteins and 25645 peptides which entailed a 46.5% of the total proteome. The C. albicans spectral library created constitutes a powerful proteomic tool available for the scientific community and could be useful for global (DIA) or targeted (SRM and PRM) studies. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-30 |
AnnouncementXML | Submission_2022-02-16_03:23:01.874.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Eduardo Romeu |
SpeciesList | scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-05 23:03:23 | ID requested | |
1 | 2021-09-30 09:39:15 | announced | |
⏵ 2 | 2022-02-16 03:23:02 | announced | 2022-02-16: Updated publication reference for PubMed record(s): 34609160. |
Publication List
Amador-Garc, í, a A, Zapico I, Borrajo A, Malmstr, ö, m J, Monteoliva L, Gil C, Extending the Proteomic Characterization of Candida albicans Exposed to Stress and Apoptotic Inducers through Data-Independent Acquisition Mass Spectrometry. mSystems, 6(5):e0094621(2021) [pubmed] |
Keyword List
submitter keyword: Candida albicans, DDA MS, spectral library, DIA MS, hydrogen peroxide, acetic acid, macrophages |
Contact List
Concha Gil García |
contact affiliation | Concha Gil, Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain |
contact email | conchagil@ucm.es |
lab head | |
Eduardo Romeu |
contact affiliation | UCM |
contact email | eromeu@ucm.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020195 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020195
- Label: PRIDE project
- Name: Creation of a Candida albicans spectral library by Data Dependent Acquisition (DDA)