PXD020183-1

PXD020183 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic Survey of Human iPSC-derived Lung Alveolar Type 2 Cells Reveals Cytopathogenic Host Responses to SARS-CoV-2 Infection
Description	Human transmission of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), the causative pathogen of the coronavirus disease 2019 (COVID-19) pandemic, is exerting a massive health and socioeconomic burden. The virus infects pneumocytes, also known as alveolar epithelial type 2 (AT2) cells, leading to impaired gas exchange and acute lung injury, but the mechanisms driving infection and pathology are unclear. Here, we report a quantitative phosphoproteomic time-course survey of a validated human AT2 cell model, derived from induced pluripotent stem cells (iPSCs), that reveals rapid, profound and switch-like rewiring of lung cell functional modules upon SARS-CoV-2 infection. Maladaptive responses driven by viral propagation include altered host cell signaling pathways, remodeled host translational processes, impaired RNA processing, cytoskeletal-microtubule disruption coincident with interphase arrest, and a pronounced innate immune response. Our study provides a data-rich resource that defines the native host systems hijacked by SARS-CoV-2 and potential therapeutic avenues for COVID-19.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:40:27.867.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ryan Hekman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-03 07:21:29	ID requested
⏵ 1	2024-10-08 11:40:28	announced

Dataset with its publication pending

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: SARS-CoV-2, mass spectrometry, human alveolar cells, drug targets, infection, phosphoproteomics, COVID-19, signaling, host pathways, pathogenesis, lung organoids

Andrew Emili
contact affiliation	Center for Network Systems Biology, Boston University
contact email	aemili@bu.edu
lab head
Ryan Hekman
contact affiliation	Boston University
contact email	rhekman@bu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD020183

PRIDE project URI

• PRIDE
  1. PXD020183
     1. Label: PRIDE project
     2. Name: Phosphoproteomic Survey of Human iPSC-derived Lung Alveolar Type 2 Cells Reveals Cytopathogenic Host Responses to SARS-CoV-2 Infection