PXD020179-1

PXD020179 is an original dataset announced via ProteomeXchange.

Title	Characterisation of FAK- and ILK-dependent nuclear subproteomes
Description	In addition to central functions in cell adhesion signalling, integrin-associated proteins have wider roles at sites distal to adhesion receptors. In experimentally defined adhesomes, there is clear enrichment of proteins that localise to the nucleus, and conversely, we now report that nuclear proteomes contain a subset of adhesome components that localise to the nucleus of cancer cells. We have defined a nucleo-adhesome, providing evidence for the remarkable scale of nuclear localisation of adhesion proteins, establishing a framework for interrogating nuclear adhesion protein functions. In addition to focal adhesion kinase (FAK), which has well-known nuclear roles, we now show that integrin-linked kinase (ILK) is another example of a biologically important nuclear adhesion protein: ILK is needed to keep squamous cell carcinoma (SCC) cancer cells alive under stress.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_02:41:16.071.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adam Byron
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	carbamoylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-03 03:59:36	ID requested
⏵ 1	2022-06-09 02:41:17	announced
2	2023-11-14 09:01:09	announced	2023-11-14: Updated project metadata.

Byron A, Griffith BGC, Herrero A, Loftus AEP, Koeleman ES, Kogerman L, Dawson JC, McGivern N, Culley J, Grimes GR, Serrels B, von Kriegsheim A, Brunton VG, Frame MC, Characterisation of a nucleo-adhesome. Nat Commun, 13(1):3053(2022) [pubmed]

submitter keyword: human, cancer, nucleus, adhesion, FAK, ILK

Margaret Frame
contact affiliation	University of Edinburgh
contact email	m.frame@ed.ac.uk
lab head
Adam Byron
contact affiliation	University of Edinburgh
contact email	adam.byron@igmm.ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD020179

PRIDE project URI

• PRIDE
  1. PXD020179
     1. Label: PRIDE project
     2. Name: Characterisation of FAK- and ILK-dependent nuclear subproteomes