PXD020151-1

PXD020151 is an original dataset announced via ProteomeXchange.

Title	Biliary Tract Carcinogenesis Model Based on Bile Metaproteomics
Description	Purpose: To analyze human and bacteria proteomic profiles in bile, exposed to a tumor vs. non-tumor microenvironment, in order to identify differences between these conditions, which may contribute to a better understanding of pancreatic carcinogenesis. Patients and Methods: Using liquid chromatography and mass spectrometry, human and bacteria proteomic profiles of a total of 20 bile samples (7 from gallstone (GS) patients, and 13 from pancreatic head ductal adenocarcinoma (PDAC) patients) that were collected during surgery, and taken directly from the gallbladder were compared. g:Profiler and KEGG (Kyoto Encyclopedia of Genes and Genomes) Mapper Reconstruct Pathway was used as the main comparative platform focusing on over-represented biological pathways among human proteins and interaction pathways among bacterial proteins. Results: Three bacterial infection pathways were over-represented in the human PDAC group of proteins. IL-8 is the only human protein that coincides in the three pathways and this protein is only present in the PDAC group. Quantitative and qualitative differences in bacterial proteins suggest a dysbiotic microenvironment in the PDAC group, supported by significant participation of antibiotic biosynthesis enzymes. Prokaryote interaction signaling pathways highlight the presence of zeatin in the GS group and surfactin in the PDAC group, the former in the metabolism of terpenoids and polyketides, and the latter in both metabolisms of terpenoids, polyketides and quorum sensing. Based on our findings, we propose a bacterial-induced carcinogenesis model for the biliary tract. Conclusion: To the best of our knowledge this is the first study with the aim of comparing human and bacteria bile proteins in a tumor vs. non-tumor microenvironment. We proposed a new carcinogenesis model for the biliary tract based on bile metaproteomic findings. Our results suggest that bacteria may be key players in biliary tract carcinogenesis, in a long-lasting dysbiotic and epithelially harmful microenvironment, in which specific bacterial species biofilm formation is of utmost importance. Our finding should be further explored in future using in vitro and in vivo investigations
HostingRepository	PRIDE
AnnounceDate	2020-07-08
AnnouncementXML	Submission_2020-07-07_22:45:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ariel Arteta
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-02 02:15:10	ID requested
⏵ 1	2020-07-07 22:45:19	announced

Dataset with its publication pending

submitter keyword: pancreatic cancer, metaproteomic, proteomic, bile, zeatin, surfactin, IL-8, carcinogenesis model

Miryan Sánchez-Jiménez, Nora Cardona-Castro
contact affiliation	Instituto Colombiano de Medicina Tropical (ICMT), Colombia
contact email	msanchez@ces.edu.co
lab head
Ariel Arteta
contact affiliation	Universidad de Antioquia Universidad CES
contact email	arteta.antonio@uces.edu.co
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020151
     1. Label: PRIDE project
     2. Name: Biliary Tract Carcinogenesis Model Based on Bile Metaproteomics