PXD020135-1

PXD020135 is an original dataset announced via ProteomeXchange.

Title	Time-resolved combined analysis of transcriptome and proteome of the pathogenicity-activated plant pathogen Xanthomonas oryzae pv. oryzae
Description	Xanthomonas oryzae pv. oryzae (Xoo) is a rice pathogen causing bacterial blight, which outbreaks in most rice cultivating countries and reduces yield up to 50% due to no effective pesticide. Urgent responses of Xoo upon the initial contacts with rice at infection site are essential for pathogenesis. We studied the time-resolved gene expression of both transcriptome and proteome in the pathogenicity-activated Xoo cells with an in vitro assay system. Genes related to cell mobility, inorganic ion transport and effectors are early response genes to help Xoo cells invade into damaged rice leaf tissues, obtain rare cofactors, and evade rice immune responses. Although the time-resolved gene expression pattern of Xoo is conserved in both mRNA and protein, there are varied time gaps in genes between the expression peaks of mRNA and protein, which implies there is an additional translational selection step of specific mRNAs for rapid translation. The expression pattern of genes from a polycistronic mRNA in the same gene cluster is strictly conserved. The time-resolved gene expression study of Xoo in both transcriptome and proteome provides a valuable information about the pathogenic responses of Xoo at the initial stage of Xoo-rice interaction.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_03:17:37.621.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wooyoung Jang
SpeciesList	scientific name: Xanthomonas oryzae pv. oryzae KACC 10331; NCBI TaxID: 291331;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-01 05:53:24	ID requested
⏵ 1	2021-09-09 03:17:38	announced
2	2024-10-22 05:28:38	announced	2024-10-22: Updated project metadata.

Kim S, Jang WE, Park J, Kim MS, Kim JG, Kang LW, . Front Microbiol, 12():664857(2021) [pubmed]

submitter keyword: Xoo–rice interactions, proteome, transcriptome, time-resolved gene expression, Xanthomonas oryzae pv. oryzae, pathogenicity

Min-Sik Kim
contact affiliation	DGIST, Department of new biology, Q-Bio laboratory
contact email	mkim@dgist.ac.kr
lab head
Wooyoung Jang
contact affiliation	kyung hee university
contact email	jangwooyoung92@khu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020135
     1. Label: PRIDE project
     2. Name: Time-resolved combined analysis of transcriptome and proteome of the pathogenicity-activated plant pathogen Xanthomonas oryzae pv. oryzae