PXD020098-1

PXD020098 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Biotin-modified cardiac-hypertrophy-associated piRNA (CHAPIR) pulldown proteins
Description	PIWI-interacting RNAs (piRNAs) are abundantly expressed during cardiac hypertrophy development, but their influence on pathological hypertrophy and the underlying mechanisms remains to be elucidated. Here, we identified a cardiac-hypertrophy-associated piRNA (CHAPIR), and we found that it regulates pathological hypertrophy. To investigate the molecular mechanisms by which CHAPIR regulates cardiomyocyte hypertrophy, we transfected the biotinylated CHAPIR into cardiomyocytes and performed streptavidin bead pull down assay. The CHAPIR pull-down materials and its control were resolved using SDS-PAGE gel and stained with coomassie blue. Then the entire gel lanes of the CHAPIR pull-down materials and its control were excised and send for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.
HostingRepository	PRIDE
AnnounceDate	2024-07-04
AnnouncementXML	Submission_2024-07-03_19:40:09.926.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fang Liu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-29 05:43:45	ID requested
⏵ 1	2024-07-03 19:40:10	announced

Publication List

10.1038/S41556-020-0576-Y;

10.1038/S41556-020-0576-Y;

Keyword List

submitter keyword: CHAPIR, piRNA,cardiac hypertrophy

submitter keyword: CHAPIR, piRNA,cardiac hypertrophy

Contact List

Kun Wang
contact affiliation	Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, College of Medicine, Qingdao University
contact email	wangk20150812@sina.com
lab head
Fang Liu
contact affiliation	Guilin Medical University
contact email	fang.liu@msn.com
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD020098

PRIDE project URI

Repository Record List

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