PXD020007-2

PXD020007 is an original dataset announced via ProteomeXchange.

Title	The CNS myelin proteome: Deep profile and persistence after post-mortem delay
Description	We revisited the proteome of myelin biochemically purified from the brains of healthy c56Bl/6N-mice utilizing complementary proteomic approaches for deep qualitative and quantitative coverage. A data-independent acquisition (DIA) workflow with alternating low and elevated energy (MSE) provided the high dynamic range required for correct quantification of highest-abundance proteins. According to this analysis, the most abundant myelin proteins PLP, MBP, CNP and MOG constitute 38%, 30%, 5% and 1% of the total myelin protein, respectively. Combined with data from an ion mobility-enhanced version of the MSE mode (referred to as UDMSE) and from 1D-gel-based proteomic approaches, we provide the most comprehensive proteome resource of CNS myelin so far and correlate it with published datasets on mRNA and protein abundance profiles of myelin and oligodendrocytes. Using dynamic range enhancement (DRE)-UDMSE as a dedicated data acquisition mode for routine differential myelin proteome profiling, we also establish that the myelin proteome displays only minor changes if assessed after a post mortem-delay of six hours. Together, these data provide a basis for addressing proteomic heterogeneity of myelin in mouse models and human patients with white matter disorders.
HostingRepository	PRIDE
AnnounceDate	2020-07-16
AnnouncementXML	Submission_2020-09-27_23:21:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-25 01:07:44	ID requested
1	2020-07-15 19:55:38	announced
⏵ 2	2020-09-27 23:21:37	announced	2020-09-28: Updated publication reference for PubMed record(s): 32973451.
3	2024-10-22 05:09:10	announced	2024-10-22: Updated project metadata.

Jahn O, Siems SB, Kusch K, Hesse D, Jung RB, Liepold T, Uecker M, Sun T, Werner HB, The CNS Myelin Proteome: Deep Profile and Persistence After Post-mortem Delay. Front Cell Neurosci, 14():239(2020) [pubmed]

submitter keyword: Oligodendrocyte, myelin proteome, central nervous system (CNS), demyelination, post mortem-delay, Autopsy, Label-free Proteomics, Transcriptome, Proteolipid protein (PLP), myelin basic protein (MBP), Cyclic nucleotide phosphodiesterase (CNP), Mass Spectrometry

PD Dr. Olaf Jahn
contact affiliation	Proteomics Group Max-Planck-Institute of Experimental Medicine Hermann-Rein-Str. 3 37075 Goettingen, Germany Tel: +49-551-3899313 Fax: +49-551-3899323 E-mail: jahn@em.mpg.de URL: www.em.mpg.de/proteomics
contact email	jahn@em.mpg.de
lab head
Olaf Jahn
contact affiliation	Proteomics Group, Max-Planck-Institute of Experimental Medicine, Göttingen
contact email	jahn@em.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD020007

PRIDE project URI

• PRIDE
  1. PXD020007
     1. Label: PRIDE project
     2. Name: The CNS myelin proteome: Deep profile and persistence after post-mortem delay