PXD019996-1

PXD019996 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	In-column thermostability of peptides
Description	The primary objective of this study was to revise the advantages of temperature in peptide separations using state-of-the-art columns, but more importantly, to reveal possible mechanisms elevated temperature can impair proteomic bottom-up LC-MS analyses.
HostingRepository	PRIDE
AnnounceDate	2020-11-17
AnnouncementXML	Submission_2020-11-16_16:40:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juraj Lenco
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Francisella tularensis subsp. holarctica LVS; NCBI TaxID: 376619;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-24 07:00:09	ID requested
⏵ 1	2020-11-16 16:40:06	announced
2	2024-10-22 05:15:14	announced	2024-10-22: Updated project metadata.

Publication List

Len, č, o J, Š, emlej T, Khalikova MA, Fabrik I, Š, vec F, Sense and Nonsense of Elevated Column Temperature in Proteomic Bottom-up LC-MS Analyses. J Proteome Res, 20(1):420-432(2021) [pubmed]

Len, č, o J, Š, emlej T, Khalikova MA, Fabrik I, Š, vec F, Sense and Nonsense of Elevated Column Temperature in Proteomic Bottom-up LC-MS Analyses. J Proteome Res, 20(1):420-432(2021) [pubmed]

Keyword List

submitter keyword: column temperature
peptide stability
peptide degradation
artificial modifications

submitter keyword: column temperature

peptide stability

peptide degradation

artificial modifications

Contact List

Juraj Lenco, Ph.D.
contact affiliation	Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Akademika Heyrovského 1203/8 500 05 Hradec Králové, Czech Republic
contact email	lenco@faf.cuni.cz
lab head
Juraj Lenco
contact affiliation	Faculty of Pharmacy, Charles University
contact email	lenco@faf.cuni.cz
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD019996
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD019996

PRIDE project URI

Repository Record List

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