PXD019977-1

PXD019977 is an original dataset announced via ProteomeXchange.

Title	Phactr1/PP1 phosphatase substrates in mouse NIH3T3 fibroblasts
Description	It was previously shown that expression of an activated Phactr1 mutant (Phactr1XXX) that constitutively forms the Phactr1/PP1 phosphatase holoenzyme, induces F-actin rearrangements in NIH3T3 fibroblasts. Expression of the Phactr1 PP1-binding domain (C-terminal) alone is also sufficient to induce such cytoskeletal changes. In contrast, expression of Phactr1XXXC derivative, which lacks the PP1 binding sequences does not result in alteration of cytoskeletal morphology (Wiezlak et al., 2012). These observations suggest that Phactr1/PP1 dephosphorylates target proteins involved in cytoskeletal dynamics. To identify potential Phactr1/PP1 substrates, we used differential SILAC phosphoproteomics in NIH3T3 cells inducibly expressing Phactr1XXX, Phactr1XXXC constructs or vector alone. Over 3000 phosphorylation sites were quantified, among which we determined Phactr1/PP1 target dephosphorylation sites by comparative analysis.
HostingRepository	PRIDE
AnnounceDate	2020-10-28
AnnouncementXML	Submission_2020-10-28_06:32:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roman Fedoryshchak
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-23 18:15:01	ID requested
⏵ 1	2020-10-28 06:32:46	announced
2	2024-10-22 05:14:20	announced	2024-10-22: Updated project metadata.

Fedoryshchak RO, P, ř, echov, á M, Butler AM, Lee R, O'Reilly N, Flynn HR, Snijders AP, Eder N, Ultanir S, Mouilleron S, Treisman R, Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme. Elife, 9():(2020) [pubmed]

submitter keyword: phosphatase, Phactr1, mouse, fibroblasts

Richard Treisman
contact affiliation	Director of Research Head, Signalling and Transcription Group The Francis Crick Institute, 1 Midland Road, London, UK
contact email	richard.treisman@crick.ac.uk
lab head
Roman Fedoryshchak
contact affiliation	The Francis Crick Institute
contact email	roman.fedoryshchak@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD019977

PRIDE project URI

• PRIDE
  1. PXD019977
     1. Label: PRIDE project
     2. Name: Phactr1/PP1 phosphatase substrates in mouse NIH3T3 fibroblasts