PXD019975-2

PXD019975 is an original dataset announced via ProteomeXchange.

Title	Porcine intestinal epithelial cells
Description	Porcine deltacoronavirus (PDCoV) is an emerging pathogen of swine belonging to family Coronaviridae, genus Deltacoronavirus. PDCoV predominantly infects the porcine intestinal epithelial cells (IPECs) causing severe diarrhea and/or vomiting, dehydration, and death in piglets. However, there are no researches for clarifying the changes of proteins expression levels in the IPECs infected with PDCoV. To better understand the host response to PDCoV infection, in this study, an isobaric tags for relative and absolute quantification (iTRAQ) labeling combined with liquid chromatography-tandem mass spectrometry (LC-MS)-based quantitative proteomic analysis of PDCoV-infected IPEC-J2 cells were performed to investigate the differentially expressed cellular proteins in the PDCoV-infected IPEC-J2 cells. As a result, a total of 5,502 host proteins were quantified at 24 hours post-infection (hpi) in mock and infected cells, among which 78 cellular proteins were differentially expressed with 23 up-regulated proteins and 55 down-regulated proteins. Bioinformatics analysis demonstrated that most of these regulated proteins participated in immune system process and structural molecule activity. Further, expression levels of two representative proteins, ANAPC7 and IFIT1, were confirmed by relative real-time RT-PCR and western blot analysis. The data presented here will provide an overview of host cell response to PDCoV, which could benefit the development of potential antiviral research.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:13:41.244.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinrong Zhou
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-23 18:14:14	ID requested
1	2020-10-19 01:09:29	announced
⏵ 2	2024-10-22 05:13:42	announced	2024-10-22: Updated project metadata.

Zhou X, Zhou L, Ge X, Guo X, Han J, Zhang Y, Yang H, Quantitative Proteomic Analysis of Porcine Intestinal Epithelial Cells Infected with Porcine Deltacoronavirus Using iTRAQ-Coupled LC-MS/MS. J Proteome Res, 19(11):4470-4485(2020) [pubmed]

10.1021/acs.jproteome.0c00592;

submitter keyword: porcine intestine LC-MS/MS

Hanchun Yang
contact affiliation	China agricultural university, preventive veterinary medicine
contact email	yanghanchun1@cau.edu.cn
lab head
Xinrong Zhou
contact affiliation	China agricultural university,
contact email	645181307@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019975
     1. Label: PRIDE project
     2. Name: Porcine intestinal epithelial cells