PXD019953-1

PXD019953 is an original dataset announced via ProteomeXchange.

Title	(p)ppGpp controls stringent factors by exploiting antagonistic allosteric coupling between catalytic domains
Description	Escherichia coli RelA is a ribosomal factor with strong (p)ppGpp synthesis activity that is dramatically activated in the presence of deacylated tRNA in the ribosomal A-site. RelA is a unique enzyme in that it is directly positively regulated by its product, alarmone nucleotide (p)ppGpp. Using HDX-MS, mulecular docking, biochemsitry, and microbiology approaches, we localise the (p)ppGpp binding site of E. coli RelA and uncover the molecular mechanism of RelA regulation by the alarmone.
HostingRepository	PRIDE
AnnounceDate	2021-08-11
AnnouncementXML	Submission_2021-08-11_05:28:45.940.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simon Ekström
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-22 07:53:51	ID requested
⏵ 1	2021-08-11 05:28:46	announced
2	2024-10-22 05:24:34	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS)

submitter keyword: Escherichia coli / stringent response / RelA / (p)ppGpp

Vasili Hauryliuk
contact affiliation	Department of Molecular Biology Umeå University SE-901 87 Umeå Sweden
contact email	vasili.hauryliuk@umu.se
lab head
Simon Ekström
contact affiliation	BioMS - Swedish National Infrastructure for Biological Mass Spectrometry, Lund University, BMC D1330, 221 84 Lund, Sweden
contact email	simon.ekstrom@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD019953

PRIDE project URI

• PRIDE
  1. PXD019953
     1. Label: PRIDE project
     2. Name: (p)ppGpp controls stringent factors by exploiting antagonistic allosteric coupling between catalytic domains