PXD019949-1

PXD019949 is an original dataset announced via ProteomeXchange.

Title	Protein posttranslational modifications of Giardia histones
Description	Diarrheal disease caused by Giardia duodenalis is highly prevalent, totalling over 200 million cases globally each year. The processes which drive parasite virulence, host immune evasion and transmission involve coordinated gene expression, which are likely post-transcriptionally regulated. Epigenetic regulatory systems are eukaryote-conserved, including in deep-branching excavates such as Giardia, with several studies already implicating histone post-translational modifications (PTMs) in regulation of its pathogenesis and life cycle. However, further insights into Giardia chromatin dynamics have been hindered by a lack of site-specific data. Using mass spectrometry, we have provided the first molecular map of histone methylation, acetylation and phosphorylation modifications in Giardia core histones. We have identified over 50 previously unreported histone modifications, including recognised epigenetic sites, combinatorial modifications indicative of PTM crosstalk, and co-occurring modifications previously correlated in model eukaryotes. These demonstrate a conserved histone code in Giardia that is equivalent to many other eukaryotes, and suggest that similar epigenetic mechanisms are in place in this parasite. Further, we used sequence, domain and structural homology to annotate putative histone enzyme networks in Giardia, highlighting representative chromatin modifiers which appear sufficient for identified sites, particularly those from H3 and H4 variants. This study is the first and most comprehensive, complete and accurate view of Giardia histone PTMs to date, and a substantial step towards understanding their associations in parasite development and virulence.
HostingRepository	PRIDE
AnnounceDate	2025-08-28
AnnouncementXML	Submission_2025-08-28_01:54:52.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samantha Emery
SpeciesList	scientific name: Giardia intestinalis assemblage A; NCBI TaxID: 941442;
ModificationList	monomethylated residue; phosphorylated residue; acetylated residue; N6; monohydroxylated residue; N6; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-22 07:53:32	ID requested
⏵ 1	2025-08-28 01:54:53	announced

Emery-Corbin SJ, Hamey JJ, Balan B, Rojas-L, ó, pez L, Sv, ä, rd SG, Jex AR, Eukaryote-conserved histone post-translational modification landscape in Giardia duodenalis revealed by mass spectrometry. Int J Parasitol, 51(4):225-239(2021) [pubmed]

10.1016/j.ijpara.2020.09.006;

submitter keyword: Methylation,Giardia, Histones, Chromatin, Acetylation, Phosphorylation

A/Prof Aaron R. Jex
contact affiliation	1 Population Health and Immunity Division, The Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, Australia Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, Australia
contact email	jex.a@wehi.edu.au
lab head
Samantha Emery
contact affiliation	Population Health and Immunity, Walter and Eliza Hall Institute of Medical Research
contact email	emery.s@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD019949

PRIDE project URI

• PRIDE
  1. PXD019949
     1. Label: PRIDE project
     2. Name: Protein posttranslational modifications of Giardia histones