PXD019922 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mesoplasma florum proteome analysis by 2D LC-MS/MS |
Description | The near-minimal bacterium Mesoplasma florum constitutes an interesting model for synthetic genomics and systems biology studies due to its small genome, fast growth rate, and lack of pathogenic potential. However, some fundamental aspects of its biology remain largely unexplored. Here, we report a broad yet remarkably detailed characterization of M. florum by combining a wide variety of experimental approaches. More specifically, we investigated several physical and physiological aspects of this bacterium, and performed the first genome-wide analysis of its transcriptome and proteome using RNA sequencing techniques and two-dimensional liquid chromatography-tandem mass spectrometry. These analyses notably revealed the M. florum consensus promoter, a first experimental cartography of transcription units, as well as the transcription and expression levels of all annotated genes. We also converted gene transcription and expression levels into absolute molecular abundances using biomass quantification results, generating an unprecedented view of the M. florum cellular composition and functions. These characterization efforts will provide an experimental foundation for the development of a genome-scale metabolic model for M. florum and will guide future genome engineering endeavours in this simple organism. |
HostingRepository | PRIDE |
AnnounceDate | 2020-11-16 |
AnnouncementXML | Submission_2020-11-16_07:56:30.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD019922 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Sébastien Rodrigue |
SpeciesList | scientific name: Mesoplasma florum L1; NCBI TaxID: 265311; |
ModificationList | phosphorylated residue; acetylated residue; formylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-21 23:38:08 | ID requested | |
⏵ 1 | 2020-11-16 07:56:30 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Mesoplasma florum, Mollicutes, 2D LC-MS/MS |
Contact List
Sébastien Rodrigue |
contact affiliation | Université de Sherbrooke, Département de biologie 2500 boul. de l'Université, Sherbrooke (Québec), Canada J1K 2R1 |
contact email | Sebastien.Rodrigue@usherbrooke.ca |
lab head | |
Sébastien Rodrigue |
contact affiliation | Université de Sherbrooke |
contact email | Sebastien.rodrigue@usherbrooke.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019922
- Label: PRIDE project
- Name: Mesoplasma florum proteome analysis by 2D LC-MS/MS