PXD019897 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cdc48A pulldown from an amino lonk column baited with SAMP1 |
Description | H. volcanii Cdc48a, RecJ3/4 and RNase J associate with Ubl-coated beads. A) Strategy to isolate H. volcanii proteins that bind agarose beads charged with monomeric Ubl (vs. BSA) as bait. Cell lysate was from triplicate cultures of H. volcanii NH02-pJAM957. B) Left, Non-reducing SDS-PAGE of H. volcanii proteins that bound BSA- (control, lane 1) vs. Ubl- (lane 2) decorated beads in the presence of ATP. Gels were stained with SYPRO Ruby. Red bar, HMW(250kDa) and LMW(50kDa) (high and low molecular weight) regions of gel excised from lanes 1 and 2 for LC- MS/MS analysis. Right, Proteins identified at > 99.9% probability and < 0.1 % false discovery rate (FDR) in the Ubl (not BSA) samples. Similar trend was observed in independent experiment. ATP was required for this association. Theor. Mr, theoretical molecular mass based on genome sequence. Normalized total spectra of sample minus control is indicated. |
HostingRepository | PRIDE |
AnnounceDate | 2023-06-22 |
AnnouncementXML | Submission_2023-06-22_14:32:13.916.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD019897 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | SwathiDantuluri |
SpeciesList | scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap XL |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-19 03:05:52 | ID requested | |
⏵ 1 | 2023-06-22 14:32:14 | announced | |
Publication List
Keyword List
submitter keyword: Cdc48A, SAMP1, HVO_2380 |
Contact List
Julie MaupinFurlow |
contact affiliation | Professor, Microbiology and Cell Science at University Of Florida. |
contact email | jmaupin@ufl.edu |
lab head | |
SwathiDantuluri |
contact affiliation | Memorial Sloan Kettering Cancer CE |
contact email | dantulus@mskcc.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD019897 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019897
- Label: PRIDE project
- Name: Cdc48A pulldown from an amino lonk column baited with SAMP1