PXD019832-1

PXD019832 is an original dataset announced via ProteomeXchange.

Title	Temporal proteomic analysis unravels the hypoxic response of melanoma cells
Description	Hypoxia is a common feature in various solid tumors including melanoma. Cancer cells in hypoxic environments are resistant to both chemotherapy and radiation. Hypoxia is also associated with immune suppression. Identification of proteins and pathways that regulate survival of cancer cells in hypoxic environments can reveal potential vulnerabilities that can be exploited to improve efficacy of anti-cancer therapy. We carried out global proteome and phosphoproteome profiling in melanoma cell lines to identify proteins and pathways that are induced by hypoxia. Here, using Orbitrap Fusion Mass Spectrometer for analysis and employing TMT-based quantitation, we report >7,000 proteins and >10,000 phosphosites. As expected, several proteins that are known targets of hypoxia inducible factors (HIFs) were found to be overexpressed in the hypoxic models. In addition, several metabolic enzymes showed altered expression revealing metabolic reprogramming in hypoxic conditions. Phosphoproteomic profiling revealed kinase mediated signaling pathways that are induced in hypoxic conditions. Our data provides a comprehensive view of proteomic and phosphoproteomic alterations in hypoxia and reveals potential mechanisms that regulate cell survival in hypoxic environments. These mechanisms can be targeted to improve therapeutic outcomes in cancer treatment. Further, we identify the 20S proteasome as a putative therapeutic target in melanoma.
HostingRepository	PRIDE
AnnounceDate	2021-09-14
AnnouncementXML	Submission_2021-09-14_08:19:20.555.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harsha Gowda
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-17 01:47:48	ID requested
⏵ 1	2021-09-14 08:19:21	announced

Datta KK, Periasamy P, Mohan SV, Ziegman R, Gowda H, Temporal Quantitative Proteomics Reveals Proteomic and Phosphoproteomic Alterations Associated with Adaptive Response to Hypoxia in Melanoma Cells. Cancers (Basel), 13(9):(2021) [pubmed]

submitter keyword: Mass spectrometry, proteome, phosphoproteome, kinome, proteasome

Harsha Gowda
contact affiliation	QIMR Berghofer Medical Research Institute
contact email	Harsha.Gowda@qimrberghofer.edu.au
lab head
Harsha Gowda
contact affiliation	QIMR Berghofer Medical Research Institute
contact email	harsha@ibioinformatics.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019832
     1. Label: PRIDE project
     2. Name: Temporal proteomic analysis unravels the hypoxic response of melanoma cells