PXD019821-1

PXD019821 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A Marine Photosynthetic Microbial Cell Factory as a Platform for Spider Silk Production
Description	Spider dragline silk protein, major ampullate spidroin (MaSp) are mainly composed of multiple types of MaSp, such as MaSp1 and MaSp2. MaSp has a conserved primary structure comprising three domains: a repetitive central domain and nonrepetitive N-terminal and C-terminal domains. The MaSp repetitive domains are arranged in alternating blocks of polyalanine (crystalline) and glycine-rich (amorphous) sequences, which are responsible for the high tensile strength and high elasticity, respectively, of spider silk fibers. Recombinant spidroins have been successfully expressed in various hosts such as bacteria, yeasts, insects, plants, and animals. However, it is still a great challenge to produce spidroins on a large scale with a sustainable production process. In this study, we develop an economical and sustainable marine photosynthetic microbial cell factory using Rhodovulum sulfidophilum, which is a marine purple nonsulfur bacterium that is capable of producing the hydrophobic repetitive sequence of MaSp1 (1-mer, 2-mer, 3-mer and 6-mer from Nephila clavipes) using small amount of organic substance under photoheterotrophic or photoautotrophic growth conditions.
HostingRepository	jPOST
AnnounceDate	2021-06-16
AnnouncementXML	Submission_2021-06-15_08:00:05.301.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019821
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Choon Pin Foong
SpeciesList	scientific name: Rhodovulum sulfidophilum DSM 1374; NCBI TaxID: 1188256;
ModificationList	unknown modification; alpha-amino acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); L-methionine sulfoxide; S-carboxamidoethyl-L-cysteine; N-formyl-L-proline
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-16 19:30:33	ID requested
⏵ 1	2021-06-15 08:00:05	announced
2	2022-09-18 02:41:04	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Spider dragline silk protein, major ampullate spidroin (MaSp), Nephila clavipes, LC-MS/MS

submitter keyword: Spider dragline silk protein, major ampullate spidroin (MaSp), Nephila clavipes, LC-MS/MS

Contact List

Keiji Numata
lab head
Choon Pin Foong
contact affiliation	Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000876

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000876