PXD019816-1

PXD019816 is an original dataset announced via ProteomeXchange.

Title	Engineering transplantable jejunal mucosal grafts using primary patient-derived organoids for children with intestinal failure
Description	Intestinal failure (IF), following extensive anatomical or functional loss of small intestine (SI), hasdebilitating long-term consequences on children1. Priority of care is to increase the child’s length of functional intestine, jejunum in particular, to promote nutritional independence2. Here we construct autologous jejunal mucosal grafts using primary patient biomaterials. We show that organoids derived from patients can be expanded efficiently in vitro. In parallel, we generate decellularized human intestinal matrix with intact nanotopography, which form optimal biological scaffolds. Remarkably, proteomic and Raman spectroscopy analyses reveal highly analogous biochemical profiles of human SI and colon scaffolds, indicating that both can be used interchangeably as platforms for intestinal engineering. Indeed, seeding jejunal organoids onto either scaffold type reliably reconstructs grafts that exhibit several aspects of physiological jejunal function with potential to survive after transplantation. Our findings provide proof-of-concept data for engineering IF patient-specific jejunal grafts, ultimately aiding in restoration of nutritional autonomy.
HostingRepository	PRIDE
AnnounceDate	2020-09-16
AnnouncementXML	Submission_2020-09-15_22:52:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Faull
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-16 08:31:34	ID requested
⏵ 1	2020-09-15 22:52:00	announced
2	2024-10-22 05:12:18	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human, colon, small intestine, organoids, label-free, LC-MS

Bram Snijders
contact affiliation	Head of the Crick Mass Spectrometry Proteomics Platform
contact email	bram.snijders@crick.ac.uk
lab head
Peter Faull
contact affiliation	The Francis Crick Institute
contact email	peter.faull@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD019816

PRIDE project URI

• PRIDE
  1. PXD019816
     1. Label: PRIDE project
     2. Name: Engineering transplantable jejunal mucosal grafts using primary patient-derived organoids for children with intestinal failure