PXD019808 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Receptor-independent modulation of cAMP-dependent protein kinase and protein phosphatase 2A signaling in cardiac myocytes by nitroxyl |
Description | Sympathetic activation is the main driver of cardiac inotropy and lusitropy. Stimulation of the -adrenoceptor (AR) signaling cascade leads to activation of cAMP-dependent protein kinase (PKA) and subsequent cardiac protein phosphorylation, which is counteracted by the corresponding protein phosphatases 2A (PP2A). Both, kinase and phosphatase are sensitive to oxidation. For PKA, the oxidant-mediated mode of regulation has been described to occur by interdisulfide formation between type I regulatory subunits (PKA-RI). Furthermore, oxidant-mediated intradisulfide formation in the catalytic subunit of PKA and PP2A was shown to inhibit kinase or phosphatase activity, respectively. Nitroxyl donors exert positive inotropic and lusitropic effects in cardiac myocytes. |
HostingRepository | PRIDE |
AnnounceDate | 2020-10-29 |
AnnouncementXML | Submission_2020-10-29_05:43:52.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD019808 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Christoph Krisp |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-16 08:30:26 | ID requested | |
⏵ 1 | 2020-10-29 05:43:52 | announced | |
Publication List
Diering S, Stathopoulou K, Goetz M, Rathjens L, Harder S, Piasecki A, Raabe J, Schulz S, Brandt M, Pflaumenbaum J, Fuchs U, Donzelli S, Sadayappan S, Nikolaev VO, Flenner F, Ehler E, Cuello F, Receptor-independent modulation of cAMP-dependent protein kinase and protein phosphatase signaling in cardiac myocytes by oxidizing agents. J Biol Chem, 295(45):15342-15365(2020) [pubmed] |
Keyword List
submitter keyword: Rat, Nitroxyl, oxidation, phosphorylation, kinase, phosphatase, cardiac myocyte |
Contact List
Friederike Cuello |
contact affiliation | Institute of Experimental Pharmacology and Toxicology; University Medical Center Hamburg-Eppendorf |
contact email | f.cuello@uke.de |
lab head | |
Christoph Krisp |
contact affiliation | University Medical Center Hamburg-Eppendorf Institute of Clinical Chemistry and Laboratory Medicine Mass Spectrometric Proteomics |
contact email | c.krisp@uke.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019808
- Label: PRIDE project
- Name: Receptor-independent modulation of cAMP-dependent protein kinase and protein phosphatase 2A signaling in cardiac myocytes by nitroxyl