<<< Full experiment listing

PXD019788-1

PXD019788 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOrdered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit
DescriptionAPC/C-mediated proteolysis of cyclin B and securin promotes entry into anaphase, inactivating CDK1 and permitting chromosome segregation, respectively. Reduction of CDK1 activity relieves inhibition of the CDK1-opposing phosphatases PP1 and PP2A-B55 leading to dephosphorylation of substrates crucial for mitotic exit. Meanwhile, continued APC/C activity is required to target various proteins, including Aurora and Polo kinases, for degradation. Together, these activities orchestrate a complex series of events during mitotic exit. However, the relative importance of regulated proteolysis and dephosphorylation in dictating the order and timing of these events remains unclear. Using high temporal-resolution mass spectrometry, we compare the relative extent of proteolysis and protein dephosphorylation. This reveals highly-selective rapid (~5min half-life) proteolysis of cyclin B, securin and geminin at the metaphase to anaphase transition, followed by slow proteolysis (>60 min half-life) of other mitotic regulators. Protein dephosphorylation requires APC/C-dependent destruction of cyclin B and was resolved into PP1-dependent fast, intermediate and slow categories with unique sequence motifs. We conclude that dephosphorylation initiated by the selective proteolysis of cyclin B drives the bulk of changes observed during mitotic exit.
HostingRepositoryPRIDE
AnnounceDate2020-09-04
AnnouncementXMLSubmission_2020-09-04_04:14:41.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJames Holder
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-15 07:24:43ID requested
12020-09-04 04:14:41announced
22023-11-14 08:50:53announced2023-11-14: Updated project metadata.
Publication List
10.7554/eLife.59885;
Keyword List
submitter keyword: mitosis, mitotic exit, cell cycle, dephosphorylation, phospho-proteomics
Contact List
Francis Barr
contact affiliationDepartment of Biochemistry, University of Oxford
contact emailfrancis.barr@bioch.ox.ac.uk
lab head
James Holder
contact affiliationUniversity of Oxford
contact emailjames.holder@path.ox.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD019788
PRIDE project URI
Repository Record List
[ + ]