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PXD019784-2

PXD019784 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHypoxis hemerocallidea (Afrtican potato) corm, leaf and flower transcriptomics and proteomics
DescriptionThe corm of Hypoxis hemerocallidea, commonly known as the African potato, is used in traditional medicine to treat several medical conditions, such as urinary infections, benign prostate hyperplasia, inflammatory conditions and testicular tumours amongst others. The metabolites of H. hemerocallidea have been identified in several studies. More recently, the terpenoids of the plant have been identified. However, the biochemical pathways and the enzymes involved in the production of metabolites have not been characterised. In this study, total RNA extracted from the corm, leaf and flower tissues of H. hemerocallidea was sequenced on the Illumina HiSeq 2500 platform. A total of 143,549 transcripts were assembled de novo using Trinity and 107,131 transcripts were functionally annotated between the nr, GO, COG, KEGG and SWISS-PROT databases. Additionally, the proteome of the three tissues was sequenced using LC-MS/MS, revealing aspects of secondary metabolism and serving as data validation for the transcriptome. Functional annotation led to the identification of numerous terpene synthases such as nerolidol synthase, germacrene D synthase and cycloartenol synthase amongst others. Transcripts were also annotated to encode for the terpene phytoalexin momilactone A synthase. Differential expression analysis using edgeR identified 946 transcripts differentially expressed between the three tissues and revealed that the leaf upregulates linalool synthase compared to the corm and the flower tissues. The transcriptome as well as the proteome of Hypoxis hemerocallidea presented here provide a foundation for future research.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:23:16.304.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSilviu Tomescu
SpeciesList scientific name: Hypoxis hemerocallidea; NCBI TaxID: 862860;
ModificationListalkylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-15 05:06:25ID requested
12021-06-23 11:19:45announced
22024-10-22 05:23:16announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: transcriptome, secondary metabolism, terpene biosynthesis., differential expression, proteome,African potato
Contact List
Karl Rumbold
contact affiliationIndustrial Microbiology and Biotechnology Laboratory, School of Molecular and Cell Biology, University of the Witwatersrand, South Africa.
contact emailKarl.Rumbold@wits.ac.za
lab head
Silviu Tomescu
contact affiliationResearcher
contact emailsilviu.mihai.tomescu@gmail.com
dataset submitter
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Dataset FTP location
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