PXD019703-2

PXD019703 is an original dataset announced via ProteomeXchange.

Title	SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state
Description	Heterochromatin is established at the 2-cell stage progression during mouse embryonic development. Here, we show that cell cycle arrest controls heterochromatin establishment in the transition from 2-cell-embryo-like (2C-like) cells to pluripotency. Conversion of embryonic stem cells (ESCs) to 2C-like cells is marked by a reorganization of H3K9me3-heterochromatin foci, which are then reversibly formed upon re-entry into pluripotency. We characterized the chromatome of 2C-like cells by genome capture, uncovering a relationship between cell cycle regulation and the establishment of the 2C-like state. Cell cycle arrest in S-G2/M induces 2C-like cells, which in turn accumulate in G2/M after cell fate conversion. Depletion of SMARCAD1, an interactor of cell cycle regulators identified by chromatin proteomics that co-localizes with H3K9me3-heterochromatin, leads to the arrest of embryo development and loss of H3K9me3. Our findings demonstrate that tight control of cell cycle progression is important for the establishment of heterochromatin foci during the transition from 2C-like cells to pluripotency.
HostingRepository	PRIDE
AnnounceDate	2023-04-21
AnnouncementXML	Submission_2023-04-21_03:16:15.999.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	EvaBorràs
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-10 18:03:59	ID requested
1	2023-04-18 02:03:30	announced
⏵ 2	2023-04-21 03:16:16	announced	2023-04-21: Updated project metadata.
3	2023-04-28 02:27:26	announced	2023-04-28: Updated project metadata.
4	2023-11-14 08:51:07	announced	2023-11-14: Updated project metadata.

submitter keyword: LC-MSMS,ESC, chromatome

EduardSabido
contact affiliation	Center for Genomic Regulation (CRG) University Pompeu Fabra (UPF)
contact email	eduard.sabido@crg.eu
lab head
EvaBorràs
contact affiliation	UPF
contact email	eva.borras@upf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD019703

PRIDE project URI

• PRIDE
  1. PXD019703
     1. Label: PRIDE project
     2. Name: SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state