PXD019702-1

PXD019702 is an original dataset announced via ProteomeXchange.

Title	Co-IP of fHERV-K env protein and interactors
Description	This project seeks to identify protein-protein interactions with human endogenous retrovirus K envelope protein. A flag-tagged HERV-K env construct (fHERV-K env) was expressed in HEK293 cells. An anti-flag MAb was immobilized on magnetic protein G beads was used to capture fHERV-K env and interactors.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:34:19.993.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019702
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jeffrey Kowalak
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	(R)-5-oxo-1,4-tetrahydrothiazine-3-carboxylic acid; 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-10 18:03:38	ID requested
⏵ 1	2024-10-07 13:34:20	announced

10.1073/pnas.2002427117;

Wang T, Medynets M, Johnson KR, Doucet-O'Hare TT, DiSanza B, Li W, Xu Y, Bagnell A, Tyagi R, Sampson K, Malik N, Steiner J, Hadegan A, Kowalak J, O'Malley J, Maric D, Nath A, Regulation of stem cell function and neuronal differentiation by HERV-K via mTOR pathway. Proc Natl Acad Sci U S A, 117(30):17842-17853(2020) [pubmed]

10.6019/PXD019702;

submitter keyword: AP-MS, on-bead digestion, human endogenous retrovirus,Co-IP

Jeffrey A Kowalak
contact affiliation	NINDS Clinical Proteomics Unit National Institutes of Health
contact email	jkowalak@mail.nih.gov
lab head
Jeffrey Kowalak
contact affiliation	NINDS Clinical Proteomics Unit
contact email	jkowalak@mail.nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD019702

PRIDE project URI

• PRIDE
  1. PXD019702
     1. Label: PRIDE project
     2. Name: Co-IP of fHERV-K env protein and interactors