PXD019677-2

PXD019677 is an original dataset announced via ProteomeXchange.

Title	Global profiling of myristoylation in Toxoplasma gondii
Description	N-myristoylation is a ubiquitous class of protein lipidation across eukaryotes and N-myristoyl transferase (NMT) has been proposed as an attractive drug target in several pathogens. Here we describe the first global chemoproteomic analysis of protein myristoylation in Toxoplasma gondii. Through quantitative mass spectrometry coupled with validated chemoproteomic tools (cleavable capture reagents (Broncel et al., 2015, Speers and Cravatt, 2005) and NMT inhibitor (Schlott et al., 2019)) that allow for experimental validation, we confidently identified 65 myristoylated proteins. This dataset represents a large fraction of the parasite’s myristoylated proteome and a prerequisite to investigate this modification in Toxoplasma.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:08:27.217.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Malgorzata Broncel
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	myristoylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-09 10:03:13	ID requested
1	2020-07-06 07:44:56	announced
⏵ 2	2024-10-22 05:08:34	announced	2024-10-22: Updated project metadata.

Broncel M, Dominicus C, Vigetti L, Nofal SD, Bartlett EJ, Touquet B, Hunt A, Wallbank BA, Federico S, Matthews S, Young JC, Tate EW, Tardieux I, Treeck M, -myristoylated protein important for host cell penetration. Elife, 9():(2020) [pubmed]

10.7554/elife.57861;

submitter keyword: N-myristoylation, Chemical proteomics, Toxoplasma, NMT inhibition

Moritz Treeck
contact affiliation	Signalling in Apicomplexan Parasites Laboratory, The Francis Crick Institute, London NW1 1AT, UK
contact email	moritz.treeck@crick.ac.uk
lab head
Malgorzata Broncel
contact affiliation	The Francis Crick Institute
contact email	malgorzata.broncel@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD019677

PRIDE project URI

• PRIDE
  1. PXD019677
     1. Label: PRIDE project
     2. Name: Global profiling of myristoylation in Toxoplasma gondii