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PXD019677 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal profiling of myristoylation in Toxoplasma gondii
DescriptionN-myristoylation is a ubiquitous class of protein lipidation across eukaryotes and N-myristoyl transferase (NMT) has been proposed as an attractive drug target in several pathogens. Here we describe the first global chemoproteomic analysis of protein myristoylation in Toxoplasma gondii. Through quantitative mass spectrometry coupled with validated chemoproteomic tools (cleavable capture reagents (Broncel et al., 2015, Speers and Cravatt, 2005) and NMT inhibitor (Schlott et al., 2019)) that allow for experimental validation, we confidently identified 65 myristoylated proteins. This dataset represents a large fraction of the parasite’s myristoylated proteome and a prerequisite to investigate this modification in Toxoplasma.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMalgorzata Broncel
SpeciesList scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmyristoylated residue
InstrumentOrbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-09 10:03:13ID requested
12020-07-06 07:44:56announced
Publication List
Broncel M, Dominicus C, Vigetti L, Nofal SD, Bartlett EJ, Touquet B, Hunt A, Wallbank BA, Federico S, Matthews S, Young JC, Tate EW, Tardieux I, Treeck M, -myristoylated protein important for host cell penetration. Elife, 9():(2020) [pubmed]
Keyword List
submitter keyword: N-myristoylation, Toxoplasma, Chemical proteomics, NMT inhibition
Contact List
Moritz Treeck
contact affiliationSignalling in Apicomplexan Parasites Laboratory, The Francis Crick Institute, London NW1 1AT, UK
contact emailmoritz.treeck@crick.ac.uk
lab head
Malgorzata Broncel
contact affiliationThe Francis Crick Institute
contact emailmalgorzata.broncel@crick.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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