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PXD019664-2

PXD019664 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of Endogenous Kinase Substrates by Proximity Labeling Combined with Kinase Perturbation and Phosphorylation Motifs
DescriptionMass spectrometry-based phosphoproteomics can identify more than 10,000 phosphorylated sites in a single experiment. But, despite the fact that enormous phosphosite information has been accumulated in public repositories, protein kinase-substrate relationships remain largely unknown. Here, we describe a method to identify endogenous substrates of kinases by using a combination of a proximity-dependent biotin identification method, called BioID, with two other independent methods, kinase-perturbed phosphoproteomics and phosphorylation motif matching. For proof-of-concept, this approach was applied to casein kinase 2 (CK2) and protein kinase A (PKA), and identified 33 and 52 putative substrates, respectively. We also show that known cancer-associated missense mutations near phosphosites of substrates affect phosphorylation by CK2 or PKA, and thus might alter downstream signaling in cancer cells bearing these mutations. This approach extends our ability to probe physiological kinase-substrate networks by providing new methodology for large-scale identification of endogenous substrates of kinases.
HostingRepositoryjPOST
AnnounceDate2021-07-16
AnnouncementXMLSubmission_2022-09-18_02:16:36.548.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTomoya Niinae
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal; L-methionine sulfoxide
Instrumentinstrument; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-09 01:54:09ID requested
12021-07-15 17:49:30announced
22022-09-18 02:16:36announced2022-09-18: Updated FTP location.
Keyword List
submitter keyword: BioID, phosphoproteomics
Contact List
Yasushi Ishihama
lab head
Tomoya Niinae
contact affiliationKyoto university
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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