PXD019655-1

PXD019655 is an original dataset announced via ProteomeXchange.

Title	Regulation of phospholipid synthesis and vesicular trafficking in Toxoplasma gondii by protein kinase TgCDPK7
Description	Calcium Dependent Protein Kinases (CDPKs) are key effector in calcium signaling in Apicomplexan parasites. CDPK7 is one such kinase, which plays a crucial role in intracellular infection of Toxoplasma gondii parasite. To gain insights into how CDPK7 play a role in parasites survival, we have performed comparative phosphoproteomic to identify putative targets of TgCDPK7 in the parasites. Proteins from TgCDPK7 knockdown and wild were isolated, digested with trypsin and labelled with TMT. From the quantitative proteomic analysis by high- resolution mass spectrometry, differentially phosphorylated proteins were identified, which can be direct or indirect substrates of TgCDPK7. Many proteins which are involved in lipid metabolism are also found significantly altered.
HostingRepository	PRIDE
AnnounceDate	2021-04-28
AnnouncementXML	Submission_2021-04-28_01:22:29.717.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keshava Prasad T. S.
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-08 07:46:02	ID requested
⏵ 1	2021-04-28 01:22:30	announced
2	2021-04-28 01:23:36	announced	2021-04-28: Updated publication reference for PubMed record(s): 33635921.

Dataset with its publication pending

submitter keyword: Toxoplasma gondii, CDPK7, Quantitative Phosphoproteomics, TMT labelling

Dr. T. S. Keshava Prasad
contact affiliation	Professor and Deputy Director, Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore 575018, India
contact email	keshav@yenepoya.edu.in
lab head
Keshava Prasad T. S.
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India.
contact email	keshav@ibioinformatics.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019655
     1. Label: PRIDE project
     2. Name: Regulation of phospholipid synthesis and vesicular trafficking in Toxoplasma gondii by protein kinase TgCDPK7