PXD019647-2

PXD019647 is an original dataset announced via ProteomeXchange.

Title	PP2A-Rts1 antagonizes Rck2-mediated hyperosmotic stress signaling in yeast
Description	In Saccharomyces cerevisiae impairment of protein phosphatase PP2A-Rts1 leads to temperature and hyperosmotic stress sensitivity, yet the underlying mechanism and the scope of action of the phosphatase in the stress response remain elusive. Using quantitative mass spectrometry-based approaches we have identified a set of putative substrate proteins that show both, hyperosmotic stress- and PP2A-Rts1-dependent changes in their phosphorylation pattern. A comparative analysis with published MS-shotgun data revealed that the phosphorylation status of many of these sites is regulated by the MAPKAP kinase Rck2, suggesting a node of regulation. Detailed gel mobility shift assays and protein-protein interaction analysis strongly suggest that Rck2 activity is directly regulated by PP2A-Rts1 via a SLiM B56-family interaction motif, uncovering a previously unknown mechanism of how PP2A influences the response to hyperosmotic stress in Yeast.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:36:07.322.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Hollenstein
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-08 04:55:10	ID requested
1	2022-05-16 03:35:36	announced
⏵ 2	2024-10-22 05:36:07	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Rck2, Stress response,Phosphorylation, Hyperosmotic stress, Hog1, phosphatase, PP2A, Stress signaling, Saccharomyces cerevisiae, Rts1, Quantitative Proteomics

Wolfgang Reiter
contact affiliation	Mass Spectrometry Facility, Max Perutz Labs, University of Vienna, Vienna BioCenter, Dr. Bohr-Gasse 9, 1030 Vienna, Austria
contact email	wolfgang.l.reiter@univie.ac.at
lab head
David Hollenstein
contact affiliation	Mass Spectrometry Facility, Max Perutz Laboratories, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 7, Vienna 1030, Austria
contact email	hollenstein.david@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019647
     1. Label: PRIDE project
     2. Name: PP2A-Rts1 antagonizes Rck2-mediated hyperosmotic stress signaling in yeast