PXD019603-2

PXD019603 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial small heat-shock proteins are essential for normal growth of Arabidopsis thaliana
Description	Mitochondria play important roles in the plant stress responses and the detoxification of the reactive oxygen species generated in the electron-transport chain. Expression of genes encoding stress-related proteins such as the mitochondrial small heat shock proteins (M-sHSP) is upregulated in response to different abiotic stresses. In Arabidopsis thaliana, three M-sHSPs paralogous genes were identified, although their function under physiological conditions remains elusive. Here, we analyzed the phenotype, proteomic and metabolic profiles of the loss-of-function mutants of M-sHSPs (single, double and triple mutants) during normal plant growth. The triple mutant showed the most prominent altered phenotype at vegetative and reproductive stages without any externally applied stress. They displayed chlorotic leaves, growth arrest and low seed production. Concomitantly, they exhibited increased levels of sugars, free amino acids such as proline, citric and ascorbic acid, among other metabolites. Single and double mutants displayed intermediate phenotype suggesting a redundant function of these proteins. All single, double and triple mutants showed alteration of proteins involved in photosynthesis, mitochondrial metabolism and antioxidant defense compared to the wild-type plants. Overall, depletion of M-sHSPs causes severe impact in fundamental metabolic processes, localized in different cell compartments, leading to alterations in the correct plant growth and development.
HostingRepository	PRIDE
AnnounceDate	2021-01-28
AnnouncementXML	Submission_2021-03-17_05:04:59.435.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariela Raquel Escobar
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-05 05:02:31	ID requested
1	2021-01-27 22:15:10	announced
⏵ 2	2021-03-17 05:05:00	announced	2021-03-17: Updated publication reference for PubMed record(s): 33643342.

Escobar MR, Feussner I, Valle EM, . Front Plant Sci, 12():600426(2021) [pubmed]

submitter keyword: abiotic stress, antioxidant, germination, growth arrest, heat stress, mitochondrial protein, sHSP

Estela Marta Valle
contact affiliation	Laboratorio de Metabolismo y Señalización en Plantas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET-UNR), Rosario, Argentina
contact email	valle@ibr-conicet.gov.ar
lab head
Mariela Raquel Escobar
contact affiliation	IBR-CONICET-UNR
contact email	escobar@ibr-conicet.gov.ar
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD019603

PRIDE project URI

• PRIDE
  1. PXD019603
     1. Label: PRIDE project
     2. Name: Mitochondrial small heat-shock proteins are essential for normal growth of Arabidopsis thaliana