PXD019564 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | S-glutathionylation proteome profiling of Streptococcus mutans |
Description | S-glutathionylation is an important post-translational modification (PTM) process that targets the protein cysteine thiol by the addition of glutathione (GSH). This modification can prevent proteolysis from over-oxidation of protein cysteine residue during the condition of oxidative or nitrosative stress. Recent studies suggest that protein S-glutathionylation plays an essential role in control of cell-signaling pathways by affecting the protein function in bacteria and even humans. In this study, we investigated the impacts of S-glutathionylation on physiological regulation within Streptococcus mutans, the primary etiological agent of human dental caries. To determine S-glutathionylated proteins in bacteria, the Cys-reactive isobaric reagents iodoTMT (iodoacetyl Tandem Mass Tag) were used to label the S-glutathionylated Cys sites and anti-TMT antibody conjugated resins were used to enrich the modified peptides. Proteome profiling identified a total of 357 glutathionylated cysteines sites on 239 proteins. Functional enrichment analysis indicated that these S-glutathionylated proteins were involved in diverse important biological processes, such as pyruvate metabolism and glycolysis |
HostingRepository | PRIDE |
AnnounceDate | 2020-07-14 |
AnnouncementXML | Submission_2020-07-14_02:41:09.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xian Peng |
SpeciesList | scientific name: Streptococcus mutans UA159; NCBI TaxID: 210007; |
ModificationList | monohydroxylated residue; acetylated residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-04 04:12:25 | ID requested | |
⏵ 1 | 2020-07-14 02:41:10 | announced | |
2 | 2024-10-22 05:08:57 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: S-glutathionylation |
Streptococcus mutans |
post-translational modification |
Contact List
Peng Xian |
contact affiliation | State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China |
contact email | pengx@scu.edu.cn |
lab head | |
Xian Peng |
contact affiliation | State Key Laboratory of Oral Diseases, Sichuan University |
contact email | pengx@scu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019564
- Label: PRIDE project
- Name: S-glutathionylation proteome profiling of Streptococcus mutans