PXD019550-1
PXD019550 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Modelling mitochondrial SAM depletion in fruit flies and mice |
Description | S-adenosylmethionine (SAM) is the principle biological methyl group donor for a diverse range of substrates. It is synthesised in the cytosolic methionine cycle and shuttled throughout the cell. The mitochondrial SAM (mitoSAM) pool depends on import through the inner-membrane SAMC and supports the maturation of metabolites and mitochondrial RNAs. Mutations in SAMC in patients cause a severe metabolic crisis, however, the organellar regulation of mitoSAM and the protein methylation landscape within mitochondria are largely unknown. Using fly and mouse models, we demonstrate that titrating mitoSAM differentially effects mitochondrial function. Metabolite and iron-sulfur cluster biosynthesis are disrupted due to acutely decreased methylation potential, while prolonged mitoSAM deficiency affects fly longevity and OXPHOS stability. The herein uploaded raw data was used for total larvae and total cell proteome quantification in the established lines. Our results define the critical role of cytoplasmic SAM production for mitochondrial methylation events and highlight the indirect effect of one-carbon metabolism on cellular bioenergetics. |
HostingRepository | PRIDE |
AnnounceDate | 2021-02-22 |
AnnouncementXML | Submission_2021-02-21_23:14:58.347.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Florian Schober |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-06-03 17:13:32 | ID requested | |
⏵ 1 | 2021-02-21 23:14:59 | announced |
Publication List
Rosenberger FA, Moore D, Atanassov I, Moedas MF, Clemente P, V, é, gv, á, ri Á, Fissi NE, Filograna R, Bucher AL, Hinze Y, The M, Hedman E, Chernogubova E, Begzati A, Wibom R, Jain M, Nilsson R, K, ä, ll L, Wedell A, Freyer C, Wredenberg A, The one-carbon pool controls mitochondrial energy metabolism via complex I and iron-sulfur clusters. Sci Adv, 7(8):(2021) [pubmed] |
Keyword List
submitter keyword: SAM, mitochondria, methylation, metabolism |
Contact List
Anna Wredenberg | |
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contact affiliation | Department of Medical Biochemistry and Biophysics, Karolinska Institute, Sweden |
contact email | anna.wredenberg@ki.se |
lab head | |
Florian Schober | |
contact affiliation | MMK, Karolinska Institute |
contact email | florian.schober@ki.se |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD019550 |
PRIDE project URI |
Repository Record List
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