PXD019534-1

PXD019534 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55
Description	Protein phosphatase regulatory subunits are increasingly recognized as promising drug targets. In the absence of an existing drug, inducible degradation provides a means of predicting candidate targets. We here employed auxin-inducible degradation of S. cerevisiae PP2A regulatory subunit Cdc55 in combination with quantitative phosphoproteomics. A prevalence of hyperphosphorylated phosphopeptides and enrichment of proteins containing the PP2A consensus sequence indicates that the approach successfully identified direct PP2A-Cdc55 targets. PRM follow up of DDA-results confirmed that vacuolar amino acid transporters are among the proteins most strongly affected by Cdc55 depletion.
HostingRepository	PanoramaPublic
AnnounceDate	2021-01-27
AnnouncementXML	Submission_2021-01-27_05:50:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Plank
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
ModificationList	Acetyl; Phospho; Oxidation; Label:13C(6); Label:2H(4); Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-02 20:18:55	ID requested
⏵ 1	2021-01-27 05:50:07	announced

Plank M, Berti M, Loewith R, Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55. Proteomics, 21(1):e2000166(2021) [pubmed]

submitter keyword: targeted MS, PRM, protein phosphatase, PP2A, degron

Robbie Loewith
contact affiliation	University of Geneva
contact email	robbie.loewith@unige.ch
lab head
Michael Plank
contact affiliation	University of Geneva
contact email	plank@email.arizona.edu
dataset submitter

Panorama Public dataset URI