<<< Full experiment listing


PXD019534 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55
DescriptionProtein phosphatase regulatory subunits are increasingly recognized as promising drug targets. In the absence of an existing drug, inducible degradation provides a means of predicting candidate targets. We here employed auxin-inducible degradation of S. cerevisiae PP2A regulatory subunit Cdc55 in combination with quantitative phosphoproteomics. A prevalence of hyperphosphorylated phosphopeptides and enrichment of proteins containing the PP2A consensus sequence indicates that the approach successfully identified direct PP2A-Cdc55 targets. PRM follow up of DDA-results confirmed that vacuolar amino acid transporters are among the proteins most strongly affected by Cdc55 depletion.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMichael Plank
SpeciesList scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
ModificationListAcetyl; Phospho; Oxidation; Label:13C(6); Label:2H(4); Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-02 20:18:55ID requested
12021-01-27 05:50:07announced
Publication List
Plank M, Berti M, Loewith R, Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55. Proteomics, 21(1):e2000166(2021) [pubmed]
Keyword List
submitter keyword: targeted MS, PRM, protein phosphatase, PP2A, degron
Contact List
Robbie Loewith
contact affiliationUniversity of Geneva
contact emailrobbie.loewith@unige.ch
lab head
Michael Plank
contact affiliationUniversity of Geneva
contact emailplank@email.arizona.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI