PXD019492-3
PXD019492 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | SWATH-MS proteomic analysis of liver samples prepared from liver-specific HyD-LIR expressing mice. |
| Description | HyD-LIR-Venus consists of HyD, a short hydrophobic domain of Aplysia phosphodiesterase 4 short-form; LIR of TP53INP2; and a fluorescent protein, Venus. This protein efficiently binds to LC3-II and GABARAP-II localizing on inner membrane of autophagosomes and does not affect autophagosome formation.Liver samples prepared from liver-specific HyD-LIR expressing mice together with the control mice were analyzed by mass spectrometry (TripleTOF 5600) coupled to Ekspert nanoLC 415 system with Ekspert cHipLC (Sciex, Framingham, MA). The analysis results were searched against the UniProt database (release 2019_11) using ProteinPilot 5.0.2. software (Sciex). The library contained 2619 protein groups with the cutoff of 1% false discovery rate. |
| HostingRepository | jPOST |
| AnnounceDate | 2020-11-09 |
| AnnouncementXML | Submission_2022-09-18_02:49:17.775.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yoshiki Miura |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | S-carboxymethyl-L-cysteine; N6-acetyl-L-lysine; alpha-amino acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); 2-pyrrolidone-5-carboxylic acid (Glu); L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-06-01 06:10:56 | ID requested | |
| 1 | 2020-11-08 22:15:20 | announced | |
| 2 | 2021-02-04 16:39:11 | announced | 2021-02-04: Updated FTP location. |
| ⏵ 3 | 2022-09-18 02:49:18 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Autophagy, Selective autophagy, LC3, GABARAP |
Contact List
| Yoshiki Miura | |
|---|---|
| lab head | |
| Yoshiki Miura | |
| contact affiliation | Laboratory of Proteomics and Biomolecular Science, Research Support Center, Juntendo University Graduate School of Medicine |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000830/ |
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