PXD019491 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Reversible oxidative signaling in Chlamydomonas following nitrogen deprivation |
Description | As global temperatures climb to historic highs, the far-reaching effects of climate change have impacted agricultural nutrient availability. This has extended to low latitude oceans, where a deficit in both nitrogen and phosphorus stores has led to dramatic decreases in carbon sequestration in oceanic phytoplankton. Although Chlamydomonas reinhardtii, a freshwater model green alga, has shown drastic systems-level alterations following nitrogen deprivation, the mechanism(s) through which these alterations are triggered and regulated are unclear. This study examined the role of reversible oxidative signaling in the nitrogen stress response of C. reinhardtii. Using oxidized cysteine resin-assisted capture enrichment coupled with label-free quantitative proteomics, 7,889 unique oxidized cysteine thiol identifiers were quantified, with 231 significantly changing peptides from 184 proteins following 2 h of nitrogen deprivation. These results demonstrate proteins affected by oxidation involved in nitrogen assimilation, amino acid metabolism, photosynthesis, and other metabolic processes. An enhanced role of non-damaging oxidative pathways is observed throughout the photosynthetic apparatus that provides a framework for further analysis in phototrophs. |
HostingRepository | PRIDE |
AnnounceDate | 2020-06-28 |
AnnouncementXML | Submission_2020-06-27_17:01:59.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD019491 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Leslie Hicks |
SpeciesList | scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055; |
ModificationList | N-ethylmaleimide derivatized cysteine |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-01 04:37:21 | ID requested | |
⏵ 1 | 2020-06-27 17:02:00 | announced | |
Publication List
Smythers AL, McConnell EW, Lewis HC, Mubarek SN, Hicks LM, Photosynthetic Metabolism and Nitrogen Reshuffling Are Regulated by Reversible Cysteine Thiol Oxidation Following Nitrogen Deprivation in Chlamydomonas. Plants (Basel), 9(6):(2020) [pubmed] |
Keyword List
submitter keyword: Chlamydomonas, oxidation, LCMS, PTM, signaling |
Contact List
Leslie M. Hicks |
contact affiliation | University of North Carolina at Chapel Hill Department of Chemistry Chapel Hill, NC, USA |
contact email | lmhicks@unc.edu |
lab head | |
Leslie Hicks |
contact affiliation | University of North Carolina at Chapel Hill |
contact email | lmhicks@unc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019491 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019491
- Label: PRIDE project
- Name: Reversible oxidative signaling in Chlamydomonas following nitrogen deprivation